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Relevance to Autism

Screening of 9q33.1 copy number variants disrupting ASTN2 or both ASTN2 and TRIM32 in clinical microarray data from 89,986 individuals across 10 sites, including 64,114 subjects with neurodevelopmental disorders (NDD), in Lionel et al., 2014 found that deletions affecting both ASTN2 and TRIM32 were statistically enriched in NDD cases compared to controls (23 deletions in 64,114 cases vs. 6 in 44,085 controls; P-value 0.019); this enrichment was subsequently determined to be male-specific [22 deletions in 40,438 NDD males vs. 2 deletions in 14,953 male controls (P-value 0.024) compared to 1 deletion in 23.676 NDD females vs. 3 deletions in 18.218 female controls (P-value 0.964)]. Zhu et al., 2019 found that absence of TRIM32 resulted in impaired generation of GABAergic interneurons and autism-like behaviors in mice via suppressed mTOR signaling.

Molecular Function

The protein encoded by this gene is a member of the tripartite motif (TRIM) family and has an E3 ubiquitin ligase activity. The protein localizes to cytoplasmic bodies and has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein.

External Links

        

References

Type
Title
Type of Disorder
Associated Disorders
Author, Year
Primary
Absence of TRIM32 Leads to Reduced GABAergic Interneuron Generation and Autism-like Behaviors in Mice via Suppressing mTOR Signaling.
ASD
Support
Disruption of the ASTN2/TRIM32 locus at 9q33.1 is a risk factor in males for autism spectrum disorders, ADHD and other neurodevelopmental phenotypes.
ASD
ADHD, DD, ID, epilepsy, OCD
Support
The E3 ligase Thin controls homeostatic plasticity through neurotransmitter release repression
Support
TRIM32 Deficiency Impairs the Generation of Pyramidal Neurons in Developing Cerebral Cortex
Support
Rare genetic susceptibility variants assessment in autism spectrum disorder: detection rate and practical use.
ASD
Variant ID
Variant Type
Allele Change
Residue Change
Inheritance Pattern
Inheritance Association
Family Type
Author, Year
 GEN1142R001 
 copy_number_loss 
  
  
 Familial 
 Maternal 
  
 GEN1142R002 
 copy_number_loss 
  
  
 Familial 
 Paternal 
  
 GEN1142R003 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R004 
 copy_number_loss 
  
  
 De novo 
  
  
 GEN1142R005 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R006 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R007 
 copy_number_loss 
  
  
 Familial 
 Maternal 
  
 GEN1142R008 
 copy_number_loss 
  
  
 Familial 
 Maternal 
  
 GEN1142R009 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R010 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R011 
 copy_number_loss 
  
  
 Familial 
 Maternal 
  
 GEN1142R012 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R013 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R014 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R015 
 copy_number_loss 
  
  
 Unknown 
  
 Multiplex 
 GEN1142R016 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R017 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R018 
 copy_number_loss 
  
  
 Unknown 
  
 Simplex 
 GEN1142R019 
 copy_number_loss 
  
  
 Familial 
 Maternal 
 Multiplex 
 GEN1142R020 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R021 
 copy_number_loss 
  
  
 Familial 
 Paternal 
  
 GEN1142R022 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R023 
 copy_number_loss 
  
  
 Familial 
 Paternal 
 Simplex 
 GEN1142R024 
 copy_number_loss 
  
  
 Familial 
 Maternal 
 Unknown 
 GEN1142R025 
 copy_number_loss 
  
  
 Familial 
 Paternal 
  
 GEN1142R026 
 copy_number_loss 
  
  
 Familial 
 Maternal 
 Simplex 
 GEN1142R027 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R028 
 copy_number_loss 
  
  
 Unknown 
  
  
 GEN1142R029 
 missense_variant 
  
 p.Arg201Cys 
 Familial 
 Paternal 
  
 GEN1142R030 
 copy_number_loss 
  
  
 Familial 
 Maternal 
 Multiplex 
Chromosome
CNV Locus
CNV Type
# of studies
Animal Model
9
Duplication
 1
 
9
Deletion
 2
 
9
Deletion-Duplication
 21
 
9
Deletion-Duplication
 30
 
9
Deletion
 1
 

Model Summary

In mice, deficiency of TRIM32 results in decreased proliferation of GABAergic interneurons, increased autophagy, and autism-like behaviors in mice including decreased social interaction, increased repetitive behaviors, and hyperactivity. Increased autophagy in TRIM32 KO mice can be rescued by treatment embryonically with 3BDO, an mTOR activator. Transplantation of M/LGE progenitors or treatment postnatally with clonazepam, an agonist of the GABAA receptor, rescues the hyperexcitability and the autistic behaviors of TRIM32 KO mice.

References

Type
Title
Author, Year
Primary
Absence of TRIM32 Leads to Reduced GABAergic Interneuron Generation and Autism-like Behaviors in Mice via Suppressing mTOR Signaling.
Additional
Deficiency of TRIM32 Impairs Motor Function and Purkinje Cells in Mid-Aged Mice
Model Type: Genetic
Model Genotype: Homozygous
Mutation: The 5 kb genomic fragment containing exon 2 of TRIM32 was replaced with an SA-IRES-beta-geopA expression cassette.
Allele Type: Knockout
Strain of Origin: 129S1
Genetic Background: 129S1
ES Cell Line: Not specified
Mutant ES Cell Line: R1 ES cells
Model Source: Nicklas et al, 2012 (PMID 22299041)
Category
Entity
Quantity
Experimental Paradigm
Age at Testing
Clasping reflex2
increased
 Tail suspension test
 10m
Motor coordination and balance2
decreased
 Horizontal ladder test
 10m
Grip strength2
decreased
 Traction test
 6m
Motor learning2
decreased
 Accelerating rotarod test
 10m
Motor coordination and balance2
decreased
 Ledge test
 10m
Grip strength2
decreased
 Traction test
 10m
Motor coordination and balance2
decreased
 Vertical pole test
 10m
Grip strength2
decreased
 Wire hang test
 6m
Gait2
abnormal
 Footprint analysis
 10m
Motor coordination and balance2
decreased
 Balance beam test
 10m
Grip strength2
decreased
 Wire hang test
 10m
Dendritic architecture: spine density2
decreased
 Golgi-Cox staining
 10m
Cerebellar morphology2
decreased
 Histology
 10m
Neuronal number: purkinje cells2
decreased
 Immunohistochemistry
 6m
Dendritic architecture: dendritic tree complexity2
decreased
 Sholl analysis
 10m
Synapse density: inhibitory1
Decreased
 Electron microscopy
 5 months
Cerebellar morphology2
decreased
 Macroscopic analysis
 10m
Neuronal number: purkinje cells2
decreased
 Immunohistochemistry
 10m
Dendritic architecture: dendritic thickness2
decreased
 Golgi-Cox staining
 10m
Synapse density: inhibitory1
Decreased
 Immunohistochemistry
 3 months
Cell proliferation: neural precursors1
Decreased
 Immunohistochemistry
 E13.5
Neuronal number: interneurons1
Decreased
 Immunohistochemistry
 E18.5, 3 weeks, 4 months
Cerebellar morphology: molecular layer thickness2
decreased
 Histology
 10m
Neuronal size2
decreased
 Golgi-Cox staining
 10m
Anatomical projections and connectivity: purkinje cell-climbing fiber connections2
decreased
 Immunohistochemistry
 10m
Electroencephalogram (eeg) signature1
Increased
 Electroencephalogram (eeg)
 4-6 months
Neurotransmitter release: gaba1
Decreased
 High-performance liquid chromatography (hplc)
 4 months
Macroautophagy: neuronal1
Increased
 Western blot
 E14.5
Macroautophagy: neuronal1
Increased
 Electron microscopy
 E14.5
Spontaneous post synaptic event frequency: inhibitory currents1
Decreased
 Whole-cell voltage clamp
 3 weeks
Stereotypy1
Increased
 Y-maze test
 4-6 months
Stereotypy2
increased
 Footprint analysis
 10m
Self grooming: perseveration1
Increased
 Grooming behavior assessments
 3 weeks, 4-6 months
Social interaction1
Decreased
 Reciprocal social interaction test
 4-6 months
Social approach1
Decreased
 Three-chamber social approach test
 4-6 months
Nest building behavior1
Decreased
 Nest building assay
 4-6 months
Social memory1
Decreased
 Three-chamber social approach test
 4-6 months
Juvenile play1
Decreased
 Reciprocal social interaction test
 3 weeks
Size/growth2
increased
 Body weight measurement
 10m
Size/growth2
increased
 Body weight measurement
 6m
Spatial working memory1
Decreased
 Morris water maze test
 4-6 months
Spatial reference memory1
Decreased
 Morris water maze test
 4-6 months
Object recognition memory1
Decreased
 Novel object recognition test
 4-6 months
Protein expression level evidence2
decreased
 Western blot
 6m
Targeted expression2
decreased
 Immunohistochemistry
 10m
Protein expression level evidence2
increased
 Western blot
 10m
Signaling: mtor pathway1
Decreased
 Western blot
 E14.5
Protein expression level evidence2
decreased
 Western blot
 10m
Protein expression level evidence1
Increased
 Mass spectrometry (ms)
 E14.5
Protein expression level evidence2
 no change
 Western blot
 6m
Motor learning2
 no change
 Accelerating rotarod test
 6m
Swimming ability1
 No change
 Morris water maze test
 4-6 months
Brain anatomy1
 No change
 Histology
 4-6 months
Cerebellar foliation2
 no change
 Histology
 10m
Neuronal number2
 no change
 Immunohistochemistry
 10m
Apoptosis: brain cells1
 No change
 Immunohistochemistry
 E13.5
Spontaneous post synaptic event amplitude: inhibitory currents1
 No change
 Whole-cell voltage clamp
 3 weeks
Repetitive digging1
 No change
 Novel cage test
 3 weeks
Olfaction1
 No change
 Olfactory discrimination test
 4-6 months
Olfaction1
 No change
 Buried food test
 4-6 months
Pain or nociception1
 No change
 Hot plate test
 4-6 months
 Not Reported: Circadian sleep/wake cycle, Communications, Developmental profile, Emotion, Immune response, Maternal behavior, Physiological parameters, Seizure

 

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