| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
Motor coordination and balance1 |
Decreased
Description: nbea mutants frequently lie on their sides and are unable to balance using their pectoral fins, unlike controls.
|
General observations |
6 dpf |
| |
|
Description: nbea mutants frequently lie on their sides and are unable to balance using their pectoral fins, unlike controls.
|
|
|
| Motor phenotype |
General locomotor activity1 |
Decreased
Description: nbea mutants show decreased overall motion compared to controls.
|
General observations |
6 dpf |
| |
|
Description: nbea mutants show decreased overall motion compared to controls.
|
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Dendritic architecture: dendritic tree complexity1 |
Decreased
Description: nbea mutant shows decreased dendritic tree complexity of Mauthner neurons compared to controls. Time course analysis shows nbea is required to maintain the extensive branching and fine dendritic processes found in mature neurons.
|
Immunofluorescence staining |
Adult |
| |
|
Description: nbea mutant shows decreased dendritic tree complexity of Mauthner neurons compared to controls. Time course analysis shows nbea is required to maintain the extensive branching and fine dendritic processes found in mature neurons.
|
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Synaptic morphology1 |
Decreased
Description: nbea mutant shows decreased expression of glycine receptor at Mauther neuron synapses in the hindbrain and spinal cord, compared to controls. Ablation of M circuit led to a loss of GlyR associated with M dendrites in the hindbrain but did not affect GlyR in the CoLo/CoLo synapses in the spinal cord. At CoLo/CoLo synapses in nbea mutants GlyR was reduced, compared to controls.
|
Immunofluorescence staining |
3-14 dpf, larva |
| |
|
Description: nbea mutant shows decreased expression of glycine receptor at Mauther neuron synapses in the hindbrain and spinal cord, compared to controls. Ablation of M circuit led to a loss of GlyR associated with M dendrites in the hindbrain but did not affect GlyR in the CoLo/CoLo synapses in the spinal cord. At CoLo/CoLo synapses in nbea mutants GlyR was reduced, compared to controls.
|
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Synaptic morphology1 |
Decreased
Description: nbea mutant shows decreased expression of connexin36, a marker of electrical synapses, at sensory/Mauthner neurons and Mauthner/Commissural local neuron synapses, compared to controls. nbea mutants show decreased connexin36 staining in the M circuit, (3-14 dpf) and also at electrical synapses in the forebrain, midbrain, hindbrain and spinal cord compared to controls.
|
Immunofluorescence staining |
3 dpf, 3-14 dpf, Larva |
| |
|
Description: nbea mutant shows decreased expression of connexin36, a marker of electrical synapses, at sensory/Mauthner neurons and Mauthner/Commissural local neuron synapses, compared to controls. nbea mutants show decreased connexin36 staining in the M circuit, (3-14 dpf) and also at electrical synapses in the forebrain, midbrain, hindbrain and spinal cord compared to controls.
|
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Synaptic morphology1 |
Decreased
Description: nbea mutant shows decreased expression of the cytosolic scaffold protein ZO-1, found at electrical synapses and non-synaptic tight junctons, compared to controls. ZO-1 colocalized with Cx36 and within the M circuit.
|
Immunofluorescence staining |
Larva |
| |
|
Description: nbea mutant shows decreased expression of the cytosolic scaffold protein ZO-1, found at electrical synapses and non-synaptic tight junctons, compared to controls. ZO-1 colocalized with Cx36 and within the M circuit.
|
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Dendritic architecture: dendritic tree complexity1 |
Decreased
Description: nbea mutants cannot maintain the fine processes resulting in loss of dendritic complexity compared to controls.
|
Immunofluorescence staining |
5 dpf |
| |
|
Description: nbea mutants cannot maintain the fine processes resulting in loss of dendritic complexity compared to controls.
|
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Synaptic morphology1 |
Decreased
Description: nbea mutant shows decreased expression of the cytosolic scaffold protein Gephyrin, found at glycinergic inhibitory synapses, compared to controls.
|
Immunofluorescence staining |
Larva |
| |
|
Description: nbea mutant shows decreased expression of the cytosolic scaffold protein Gephyrin, found at glycinergic inhibitory synapses, compared to controls.
|
|
|
| Neurophysiology |
Synaptic transmission1 |
Decreased
Description: nbea mutant shows decreased electrical synapse function compared to controls.
|
Retrograde labeling assay |
Larva |
| |
|
Description: nbea mutant shows decreased electrical synapse function compared to controls.
|
|
|
| Sensory |
Startle response: acoustic stimulus1 |
Decreased
Description: nbea mutants show decreased frequency of response in acoustic startle reponse test compared to wildtype controls although mutants could perform escape responses that were indistinguishable from wildtype but responded in fewer trials.
|
Acoustic startle reflex test |
6 dpf |
| |
|
Description: nbea mutants show decreased frequency of response in acoustic startle reponse test compared to wildtype controls although mutants could perform escape responses that were indistinguishable from wildtype but responded in fewer trials.
|
|
|
| Sensory |
Touch1 |
Decreased
Description: nbea mutants show decreased responsiveness to touch compared to wildtype controls.
|
Gentle touch test |
6 dpf |
| |
|
Description: nbea mutants show decreased responsiveness to touch compared to wildtype controls.
|
|
|
| Developmental profile |
Mortality/lethality1 |
Decreased
Description: nbea mutants surivive to at least 14dpf but die before they reach adulthood.
|
General observations |
adult |
| |
|
Description: nbea mutants surivive to at least 14dpf but die before they reach adulthood.
|
|
|
| Molecular profile |
Targeted expression1 |
Decreased
Description: nbea mutant shows decreased expression of nbea protein compared to wildtype controls.
|
Immunofluorescence staining |
Larva |
| |
|
Description: nbea mutant shows decreased expression of nbea protein compared to wildtype controls.
|
|
|
| Developmental profile |
Developmental trajectory1 |
No change |
General observations |
Larva |
| Developmental profile |
Developmental trajectory1 |
No change |
Macroscopic analysis |
5 dpf |
| Developmental profile |
General characteristics1 |
No change |
General observations |
Larva |
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Anatomical projections and connectivity1 |
No change |
Immunofluorescence staining |
5 dpf |
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Anatomical projections and connectivity1 |
No change |
Immunofluorescence staining |
5 dpf |
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Dendritic architecture: dendritic tree complexity1 |
No change |
Immunofluorescence staining |
1-4 dpf |
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Neuronal number1 |
No change |
Immunofluorescence staining |
5 dpf |
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Synaptic morphology: active zone1 |
No change |
Immunofluorescence staining |
Larva |
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Synaptic morphology: active zone1 |
No change |
Immunofluorescence staining |
Larva |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Emotion, Immune response, Learning & memory, Maternal behavior, Physiological parameters, Repetitive behavior, Seizure, Social behavior, |
