| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Dendritic architecture: dendritic length1 |
Decreased
Description: Mecp2-null mutants showed a significant reduction in the mean dendrite length of trigeminal ganglion neurons compared to WT controls.
|
Immunofluorescence staining |
24 hpf |
| |
|
Description: Mecp2-null mutants showed a significant reduction in the mean dendrite length of trigeminal ganglion neurons compared to WT controls.
|
|
|
| Physiological parameters |
Digestive system function: gastrointestinal motility2 |
Decreased
Description: mecp2-null mutants showed green/yellow discoloration in the GI tracts indicative of an accumulation of, or disruption in, flow of bile compared to controls. At 7 dpf, dark yellow droplets were regularly observed in the GI tracts of mecp2-null mutants, consistent with bile overproduction.
|
General observations |
4-7 dpf |
| |
|
Description: mecp2-null mutants showed green/yellow discoloration in the GI tracts indicative of an accumulation of, or disruption in, flow of bile compared to controls. At 7 dpf, dark yellow droplets were regularly observed in the GI tracts of mecp2-null mutants, consistent with bile overproduction.
|
|
|
| Developmental profile |
Size/growth2 |
Decreased
Description: mecp2-null mutants showed growth retardation compared to controls.
|
General observations |
2 dpf |
| |
|
Description: mecp2-null mutants showed growth retardation compared to controls.
|
|
|
| Immune response |
Innate immune response2 |
Increased
Description: mecp2-null mutants showed an increase in neutrophil number compared to controls.
|
Fluorescence microscopy |
4-5 dpf |
| |
|
Description: mecp2-null mutants showed an increase in neutrophil number compared to controls.
|
|
|
| Immune response |
Innate immune response2 |
Increased
Description: mecp2-null mutants showed an increase in neutrophil number in the GI tract compared to controls.
|
Fluorescence microscopy |
2-5 dpf |
| |
|
Description: mecp2-null mutants showed an increase in neutrophil number in the GI tract compared to controls.
|
|
|
| Molecular profile |
Gene expression1 |
Decreased
Description: Mecp2-null mutants showed a reduction in expression of robo2 gene compared to controls.
|
Quantitative pcr (qrt-pcr) |
24 hpf |
| |
|
Description: Mecp2-null mutants showed a reduction in expression of robo2 gene compared to controls.
|
|
|
| Molecular profile |
Gene expression2 |
Decreased
Description: Starting at 6 hpf, when tnfa expression is detectable in wild-type controls, mecp2-null mutants showed a decrease in tnfa expression compared to controls.
|
Quantitative pcr (qrt-pcr) |
6 hpf - 7 dpf |
| |
|
Description: Starting at 6 hpf, when tnfa expression is detectable in wild-type controls, mecp2-null mutants showed a decrease in tnfa expression compared to controls.
|
|
|
| Molecular profile |
Gene expression2 |
Abnormal
Description: mecp2-null mutants showed abnormal gene expression compared to controls. Specifically, 3837 transcripts were significantly upregulated and 4217 transcripts were significantly downregulated compared to controls.
|
Rna sequencing |
6 hpf |
| |
|
Description: mecp2-null mutants showed abnormal gene expression compared to controls. Specifically, 3837 transcripts were significantly upregulated and 4217 transcripts were significantly downregulated compared to controls.
|
|
|
| Molecular profile |
Gene expression2 |
Decreased
Description: mecp2-null mutants showed a decrease in tnfa expression compared to controls.
|
Confocal microscopy |
3 dpf |
| |
|
Description: mecp2-null mutants showed a decrease in tnfa expression compared to controls.
|
|
|
| Molecular profile |
Gene expression2 |
Increased
Description: mecp2-null mutants showed an increase in il1b expression compared to controls.
|
Quantitative pcr (qrt-pcr) |
5-6 dpf |
| |
|
Description: mecp2-null mutants showed an increase in il1b expression compared to controls.
|
|
|
| Molecular profile |
Gene expression1 |
Decreased
Description: Mecp2-null mutants showed a reduction in expression of sema5b gene compared to controls.
|
Quantitative pcr (qrt-pcr) |
24 hpf |
| |
|
Description: Mecp2-null mutants showed a reduction in expression of sema5b gene compared to controls.
|
|
|
| Molecular profile |
Gene expression2 |
Decreased
Description: mecp2-null mutants showed a decrease in tnfa expression as part of innate immune response compared to controls.
|
Quantitative pcr (qrt-pcr) |
3 dpf |
| |
|
Description: mecp2-null mutants showed a decrease in tnfa expression as part of innate immune response compared to controls.
|
|
|
| Molecular profile |
Gene expression2 |
Increased
Description: mecp2-null mutants showed an increase in il10 expression compared to controls.
|
Quantitative pcr (qrt-pcr) |
5-6 dpf |
| |
|
Description: mecp2-null mutants showed an increase in il10 expression compared to controls.
|
|
|
| Molecular profile |
Gene expression1 |
Decreased
Description: Mecp2-null mutants showed a reduction in expression of sema3f gene compared to controls.
|
Quantitative pcr (qrt-pcr) |
24 hpf |
| |
|
Description: Mecp2-null mutants showed a reduction in expression of sema3f gene compared to controls.
|
|
|
| Molecular profile |
Gene expression2 |
Increased
Description: mecp2-null mutants showed an increase in crp expression compared to controls.
|
Quantitative pcr (qrt-pcr) |
4-5 dpf |
| |
|
Description: mecp2-null mutants showed an increase in crp expression compared to controls.
|
|
|
| Developmental profile |
Size/growth2 |
No change |
General observations |
7 dpf |
| Immune response |
Innate immune response2 |
No change |
Fluorescence microscopy |
3 dpf |
| Immune response |
Innate immune response2 |
No change |
Fluorescence microscopy |
5 dpf |
| Immune response |
Innate immune response2 |
No change |
Phagocytosis assay |
3 dpf |
| Immune response |
Microgliosis2 |
No change |
Fluorescence microscopy |
3-5 dpf |
| Molecular profile |
Gene expression2 |
No change |
Quantitative pcr (qrt-pcr) |
3 dpf |
| Molecular profile |
Gene expression2 |
No change |
Quantitative pcr (qrt-pcr) |
0-3 dpf |
| Molecular profile |
Gene expression2 |
No change |
Quantitative pcr (qrt-pcr) |
1-7 dpf |
| Molecular profile |
Gene expression2 |
No change |
Quantitative pcr (qrt-pcr) |
1-4, 7 dpf |
| Molecular profile |
Gene expression2 |
No change |
Quantitative pcr (qrt-pcr) |
3 dpf |
| Molecular profile |
Gene expression2 |
No change |
Quantitative pcr (qrt-pcr) |
1-4, 7 dpf |
| Molecular profile |
Gene expression2 |
No change |
Quantitative pcr (qrt-pcr) |
1-7 dpf |
| Molecular profile |
Gene expression2 |
No change |
Quantitative pcr (qrt-pcr) |
1-7 dpf |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Emotion, Immune response, Learning & memory, Maternal behavior, Motor phenotype, Neuroanatomy / ultrastructure / cytoarchitecture, Neurophysiology, Physiological parameters, Repetitive behavior, Seizure, Sensory, Social behavior, |
