| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Axon architecture: axon length1 |
Decreased
Description: Mecp2 MO morphants showed a significant reduction in total length of peripheral axon projections compared to controls.
|
Immunofluorescence staining |
24 hpf |
| |
|
Description: Mecp2 MO morphants showed a significant reduction in total length of peripheral axon projections compared to controls.
|
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Axon architecture: axon length1 |
Sustained effect
Description: Co-injection of mecp2 mRNA rescues the reduction in the total projection length of trigeminal ganglion neurons in mecp2 MO morphants.
|
Immunofluorescence staining |
24 hpf |
| |
|
Description: Co-injection of mecp2 mRNA rescues the reduction in the total projection length of trigeminal ganglion neurons in mecp2 MO morphants.
|
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Dendritic architecture: dendritic length1 |
Decreased
Description: Mecp2 MO morphants showed significantly less peripheral projections from trigeminal ganglion neurons compared to WT controls.
|
Immunofluorescence staining |
24 hpf |
| |
|
Description: Mecp2 MO morphants showed significantly less peripheral projections from trigeminal ganglion neurons compared to WT controls.
|
|
|
| Sensory |
Touch1 |
Decreased
Description: Mecp2 MO morphants showed a reduction in responseness to tactile stimulation compared to controls.
|
General observations |
48 hpf |
| |
|
Description: Mecp2 MO morphants showed a reduction in responseness to tactile stimulation compared to controls.
|
|
|
| Physiological parameters |
Apoptosis1 |
Increased
Description: Mecp2 MO morphants showed a significant increase in the level of apoptosis compared to controls.
|
Tunel assay |
16, 24 hpf |
| |
|
Description: Mecp2 MO morphants showed a significant increase in the level of apoptosis compared to controls.
|
|
|
| Molecular profile |
Gene expression1 |
Decreased
Description: Mecp2 MO morphants showed a reduction in expression of sema3f gene compared to controls.
|
Quantitative pcr (qrt-pcr) |
24 hpf |
| |
|
Description: Mecp2 MO morphants showed a reduction in expression of sema3f gene compared to controls.
|
|
|
| Molecular profile |
Gene expression1 |
Decreased
Description: Mecp2 MO morphants showed a reduction in expression of robo2 gene compared to controls.
|
Quantitative pcr (qrt-pcr) |
24 hpf |
| |
|
Description: Mecp2 MO morphants showed a reduction in expression of robo2 gene compared to controls.
|
|
|
| Molecular profile |
Gene expression1 |
Abnormal
Description: Mecp2 MO morphants showed misregulation in expression of 1123 genes with a minimum of 2-fold change compared to controls, of which 1005 (607 downregulated and 398 upregulated) had a mammalian ortholog and 118 (43 downregulated and 75 upregulated) didn't.
|
Gene expression microarray |
24 hpf |
| |
|
Description: Mecp2 MO morphants showed misregulation in expression of 1123 genes with a minimum of 2-fold change compared to controls, of which 1005 (607 downregulated and 398 upregulated) had a mammalian ortholog and 118 (43 downregulated and 75 upregulated) didn't.
|
|
|
| Molecular profile |
Gene expression1 |
Decreased
Description: Mecp2 MO morphants showed a reduction in expression of sema5b gene compared to controls.
|
Quantitative pcr (qrt-pcr) |
24 hpf |
| |
|
Description: Mecp2 MO morphants showed a reduction in expression of sema5b gene compared to controls.
|
|
|
| Developmental profile |
Muscular fiber morphology1 |
No change |
Immunohistochemistry |
Not reported |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Emotion, Immune response, Learning & memory, Maternal behavior, Motor phenotype, Neurophysiology, Physiological parameters, Repetitive behavior, Seizure, Social behavior, |
