Description: Mecp2 knockdown paws had greater numbers of sub- and intraepithelial nerve fibers. Innervation density is a result of non-peptidergic fiber proliferation.
Exp Paradigm: Immunohistochemistry

Description: Smaller force threshold for paw withdrawal.
Exp Paradigm: Following acclimation, six monofilaments, ranging from 2 to 15 g, were applied five times to each hind paw at intervals of not less than 2 min. The number of positive responses, as observed by lifting or biting of the hind paw, was recorded. The calibrated force of the monofilament generating a 50% withdrawal response rate was recorded as the withdrawal threshold.