| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
General locomotor activity: ambulatory activity1 |
Decreased
Description: Mutants travel shorter distances.
Exp Paradigm: NA
|
Open field test |
3-18 weeks |
| |
|
Description: Mutants travel shorter distances.
Exp Paradigm: NA |
|
|
| Repetitive behavior |
Self grooming: perseveration1 |
Increased
Description: Male mutants spent more time self-grooming.
Exp Paradigm: NA
|
Grooming behavior assessments |
3-18 weeks |
| |
|
Description: Male mutants spent more time self-grooming.
Exp Paradigm: NA |
|
|
| Social behavior |
Social transmission of food preference1 |
Decreased
Description: Mutants failed to acquire the food preference of another mouse following 30 min co-housing.
Exp Paradigm: NA
|
Social transmission of food preference |
3-18 weeks |
| |
|
Description: Mutants failed to acquire the food preference of another mouse following 30 min co-housing.
Exp Paradigm: NA |
|
|
| Social behavior |
Social memory1 |
Decreased
Description: Male mutants show no preference for an unfamiliar mouse over a familiar mouse.
Exp Paradigm: NA
|
Three-chamber social approach test |
3-18 weeks |
| |
|
Description: Male mutants show no preference for an unfamiliar mouse over a familiar mouse.
Exp Paradigm: NA |
|
|
| Emotion |
Depression1 |
Increased
Description: Mutants spend more time immobile.
Exp Paradigm: NA
|
Tail suspension test |
3-18 weeks |
| |
|
Description: Mutants spend more time immobile.
Exp Paradigm: NA |
|
|
| Emotion |
Anxiety1 |
Increased
Description: Mutants spent less time in the open arms.
Exp Paradigm: NA
|
Elevated plus maze test |
3-18 weeks |
| |
|
Description: Mutants spent less time in the open arms.
Exp Paradigm: NA |
|
|
| Learning & memory |
Object recognition memory1 |
Decreased
Description: Mutants spent less time playing with a novel object.
Exp Paradigm: NA
|
Novel object recognition test |
3-18 weeks |
| |
|
Description: Mutants spent less time playing with a novel object.
Exp Paradigm: NA |
|
|
| Molecular profile |
Targeted expression1 |
Decreased
Description: Male mutants show decreased mecp2 transcript expression in the hippocampus and parietal cortex.
Exp Paradigm: NA
|
Quantitative pcr (qrt-pcr) |
3-18 weeks |
| |
|
Description: Male mutants show decreased mecp2 transcript expression in the hippocampus and parietal cortex.
Exp Paradigm: NA |
|
|
| Molecular profile |
Targeted expression1 |
Decreased
Description: Male mutants show decreased mecp2 protein expression in the hippocampus and parietal cortex.
Exp Paradigm: NA
|
Immunofluorescence staining |
3-18 weeks |
| |
|
Description: Male mutants show decreased mecp2 protein expression in the hippocampus and parietal cortex.
Exp Paradigm: NA |
|
|
| Molecular profile |
Dna methylation1 |
Increased
Description: Male mutants show increased mecp2 promoter methylation in tail samples and in the parietal cortex, hippocampus, heart, liver, kidney, lung, and testis.
Exp Paradigm: NA
|
Cpg methylomics analysis |
3-18 weeks |
| |
|
Description: Male mutants show increased mecp2 promoter methylation in tail samples and in the parietal cortex, hippocampus, heart, liver, kidney, lung, and testis.
Exp Paradigm: NA |
|
|
| Molecular profile |
Dna methylation1 |
No change |
Cpg methylomics analysis |
3-18 weeks |
| Social behavior |
Social approach1 |
No change |
Three-chamber social approach test |
3-18 weeks |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Immune response, Maternal behavior, Neuroanatomy / ultrastructure / cytoarchitecture, Neurophysiology, Physiological parameters, Seizure, Sensory, |
