| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
General locomotor activity: ambulatory activity1 |
Decreased
Description: Mutants show decrease in distance travelled.
Exp Paradigm: NA
|
Open field test |
6 weeks |
| |
|
Description: Mutants show decrease in distance travelled.
Exp Paradigm: NA |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Neuronal number: interneurons1 |
Increased
Description: Mutants show increased parvalbumin positive neurons in the primary somatosensory cortex and in the visual cortex but not in the basolateral amygdala.
Exp Paradigm: NA
|
Immunohistochemistry |
6 weeks |
| |
|
Description: Mutants show increased parvalbumin positive neurons in the primary somatosensory cortex and in the visual cortex but not in the basolateral amygdala.
Exp Paradigm: NA |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Neuronal number: interneurons1 |
Decreased
Description: Mutants show decrease in the number of interneurons in the basolateral amygdala.
Exp Paradigm: NA
|
Immunohistochemistry |
6 weeks |
| |
|
Description: Mutants show decrease in the number of interneurons in the basolateral amygdala.
Exp Paradigm: NA |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Neuroreceptor levels: gaba-r: gabaa1 |
Decreased
Description: Mutants show decreased expression of gabrb3 expression in the spinal cord dorsal horn lamina iii/iv.
Exp Paradigm: Spinal cord
|
Immunohistochemistry |
6 weeks |
| |
|
Description: Mutants show decreased expression of gabrb3 expression in the spinal cord dorsal horn lamina iii/iv.
Exp Paradigm: Spinal cord |
|
|
| Neurophysiology |
Synaptic transmission1 |
Decreased
Description: Mutants show decrease in excitatory to inhibitory ratio in primary somatosensory cortex slices.
Exp Paradigm: NA
|
Whole-cell patch clamp |
6 weeks |
| |
|
Description: Mutants show decrease in excitatory to inhibitory ratio in primary somatosensory cortex slices.
Exp Paradigm: NA |
|
|
| Neurophysiology |
Spontaneous post synaptic event frequency: inhibitory currents1 |
Abnormal
Description: Mutants show increase in sipsc frequency in the primary visual cortex but not in the somatosensory cortex.
Exp Paradigm: NA
|
Whole-cell patch clamp |
6 weeks |
| |
|
Description: Mutants show increase in sipsc frequency in the primary visual cortex but not in the somatosensory cortex.
Exp Paradigm: NA |
|
|
| Neurophysiology |
Synaptic transmission1 |
Decreased
Description: Mutants show decrease in excitatory to inhibitory ratio in primary somatosensory cortex slices.
Exp Paradigm: Primary somatosensory cortex
|
Whole-cell patch clamp |
6 weeks |
| |
|
Description: Mutants show decrease in excitatory to inhibitory ratio in primary somatosensory cortex slices.
Exp Paradigm: Primary somatosensory cortex |
|
|
| Repetitive behavior |
Repetitive digging1 |
Decreased
Description: Mutants show decrease in the number of marbles buried.
Exp Paradigm: NA
|
Marble-burying test |
6 weeks |
| |
|
Description: Mutants show decrease in the number of marbles buried.
Exp Paradigm: NA |
|
|
| Sensory |
Sensorimotor gating: tactile cue1 |
Increased
Description: Mutants show increase in response to air puff.
Exp Paradigm: NA
|
Response to air puff |
6 weeks |
| |
|
Description: Mutants show increase in response to air puff.
Exp Paradigm: NA |
|
|
| Sensory |
Tactile memory1 |
Decreased
Description: Mutants show decreased preference for object with novel texture.
Exp Paradigm: NA
|
Textured novel object recognition test (t-nort) |
6 weeks |
| |
|
Description: Mutants show decreased preference for object with novel texture.
Exp Paradigm: NA |
|
|
| Sensory |
Sensorimotor gating1 |
Increased
Description: Mutants show increased percent prepulse inhibition of the startle response to a 125 db noise, when the startle noise is preceded by a prepulse at 80db.
Exp Paradigm: NA
|
Prepulse inhibition |
6 weeks |
| |
|
Description: Mutants show increased percent prepulse inhibition of the startle response to a 125 db noise, when the startle noise is preceded by a prepulse at 80db.
Exp Paradigm: NA |
|
|
| Sensory |
Startle response: acoustic stimulus1 |
Decreased
Description: Mutants show decrease in amplitude of startle response to a 125 db startling noise but not to a 80db non-startling noise pulse.
Exp Paradigm: NA
|
Acoustic startle reflex test |
6 weeks |
| |
|
Description: Mutants show decrease in amplitude of startle response to a 125 db startling noise but not to a 80db non-startling noise pulse.
Exp Paradigm: NA |
|
|
| Sensory |
Sensorimotor gating: tactile cue1 |
Increased
Description: Mutants show increased percent inhibition of the startle response to a 125 db noise, when the startle noise is preceded by a light air puff.
Exp Paradigm: NA
|
Prepulse inhibition |
6 weeks |
| |
|
Description: Mutants show increased percent inhibition of the startle response to a 125 db noise, when the startle noise is preceded by a light air puff.
Exp Paradigm: NA |
|
|
| Social behavior |
Social memory1 |
Decreased
Description: Mutants show decrease in preference for novel mouse over familiar mouse.
Exp Paradigm: NA
|
Three-chamber social approach test |
6 weeks |
| |
|
Description: Mutants show decrease in preference for novel mouse over familiar mouse.
Exp Paradigm: NA |
|
|
| Social behavior |
Social approach1 |
Decreased
Description: Mutants show decrease in the preference for novel mouse over novel object.
Exp Paradigm: NA
|
Three-chamber social approach test |
6 weeks |
| |
|
Description: Mutants show decrease in the preference for novel mouse over novel object.
Exp Paradigm: NA |
|
|
| Social behavior |
Social approach1 |
Decreased
Description: Mutants show decrease in preference for novel mouse over novel object.
Exp Paradigm: NA
|
Three-chamber social approach test |
6 weeks |
| |
|
Description: Mutants show decrease in preference for novel mouse over novel object.
Exp Paradigm: NA |
|
|
| Developmental profile |
Size/growth1 |
Decreased
Description: Mutants show reduced body weight.
Exp Paradigm: NA
|
Body weight measurement |
NA |
| |
|
Description: Mutants show reduced body weight.
Exp Paradigm: NA |
|
|
| Developmental profile |
General characteristics1 |
Decreased
Description: Mutants show dull eyes, dull/ungroomed coat and somewhat hunched stance.
Exp Paradigm: A score of 0 indicated a clean shiny coat, clear eyes and normal stance. a score of 1 indicated the appearance of dull eyes, dull/ungroomed coat and somewhat hunched stance. a score of 2 denoted narrowed or crusted eyes, piloerection and a hunched posture. a score of 3 indicated severe eye narrowing, profound piloerection or loss of fur, and severely hunched posture.
|
Macroscopic analysis |
3 weeks |
| |
|
Description: Mutants show dull eyes, dull/ungroomed coat and somewhat hunched stance.
Exp Paradigm: A score of 0 indicated a clean shiny coat, clear eyes and normal stance. a score of 1 indicated the appearance of dull eyes, dull/ungroomed coat and somewhat hunched stance. a score of 2 denoted narrowed or crusted eyes, piloerection and a hunched posture. a score of 3 indicated severe eye narrowing, profound piloerection or loss of fur, and severely hunched posture. |
|
|
| Emotion |
Habituation to aversive stimuli1 |
Decreased
Description: Mutants show decreased startle amplitude to a 125 db noise during a 30-minute tactile ppi session, when comparing the first five startle responses to the last five responses to a 125 db noise.
Exp Paradigm: NA
|
Prepulse inhibition |
6 weeks |
| |
|
Description: Mutants show decreased startle amplitude to a 125 db noise during a 30-minute tactile ppi session, when comparing the first five startle responses to the last five responses to a 125 db noise.
Exp Paradigm: NA |
|
|
| Emotion |
Exploratory activity1 |
Increased
Description: Mutants show increase in time spent investigating objects.
Exp Paradigm: NA
|
Novel object recognition test |
6 weeks |
| |
|
Description: Mutants show increase in time spent investigating objects.
Exp Paradigm: NA |
|
|
| Emotion |
Anxiety1 |
Increased
Description: Mutants show decrease in time spent in the open arms.
Exp Paradigm: NA
|
Elevated plus maze test |
6 weeks |
| |
|
Description: Mutants show decrease in time spent in the open arms.
Exp Paradigm: NA |
|
|
| Emotion |
Anxiety1 |
Increased
Description: Mutants show decrease in time spent in the center.
Exp Paradigm: NA
|
Open field test |
6 weeks |
| |
|
Description: Mutants show decrease in time spent in the center.
Exp Paradigm: NA |
|
|
| Learning & memory |
Object recognition memory: long-term recall1 |
Decreased
Description: Mutants show decrease in preference for object with novel shape after 1 hour retention but not after 5 min retention.
Exp Paradigm: NA
|
Novel object recognition test |
6 weeks |
| |
|
Description: Mutants show decrease in preference for object with novel shape after 1 hour retention but not after 5 min retention.
Exp Paradigm: NA |
|
|
| Emotion |
Exploratory activity1 |
No change |
Novel object recognition test |
6 weeks |
| Motor phenotype |
General locomotor activity1 |
No change |
Elevated plus maze test |
6 weeks |
| Neurophysiology |
Spontaneous post synaptic event amplitude: excitatory currents1 |
No change |
Whole-cell patch clamp |
6 weeks |
| Neurophysiology |
Spontaneous post synaptic event amplitude: inhibitory currents1 |
No change |
Whole-cell patch clamp |
6 weeks |
| Neurophysiology |
Spontaneous post synaptic event frequency: excitatory currents1 |
No change |
Whole-cell patch clamp |
6 weeks |
| Repetitive behavior |
Self grooming: perseveration1 |
No change |
Grooming behavior assessments |
6 weeks |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Immune response, Maternal behavior, Molecular profile, Physiological parameters, Seizure, |
