| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
Clasping reflex1 |
Increased
Description: Mecp2- pvn ko mice develop clear hind limb retraction (clasping) after 15 weeks of age
Exp Paradigm: NA
|
General observations |
5 months |
| |
|
Description: Mecp2- pvn ko mice develop clear hind limb retraction (clasping) after 15 weeks of age
Exp Paradigm: NA |
|
|
| Motor phenotype |
Ataxia1 |
Increased
Description: Mecp2-pvn ko mice have increased ataxia measured as shorter latency to fall of the accelerating rotarod and in the dowel walk test, the ataxia worsens with age and by 10 weeks the mutants also have splayed hindlegs determined by footprint analysis
Exp Paradigm: Dowel walk test
|
Dowel walk test |
6 weeks, 5 months, 2.5 months |
| |
|
Description: Mecp2-pvn ko mice have increased ataxia measured as shorter latency to fall of the accelerating rotarod and in the dowel walk test, the ataxia worsens with age and by 10 weeks the mutants also have splayed hindlegs determined by footprint analysis
Exp Paradigm: Dowel walk test |
|
|
| Motor phenotype |
Motor coordination and balance1 |
Decreased
Description: Mecp2 pvn ko mice have reduced motor coordination and balance as the latency to fall from the accelerating rotarod and dowel walk assay, authors also attribute reduced marble burying to reduced coordination in forelimbs
Exp Paradigm: Marble-burying test
|
Marble-burying test |
6 weeks, 5 months, 2.5 months |
| |
|
Description: Mecp2 pvn ko mice have reduced motor coordination and balance as the latency to fall from the accelerating rotarod and dowel walk assay, authors also attribute reduced marble burying to reduced coordination in forelimbs
Exp Paradigm: Marble-burying test |
|
|
| Motor phenotype |
Ataxia1 |
Increased
Description: Mecp2-pvn ko mice have increased ataxia measured as shorter latency to fall of the accelerating rotarod and in the dowel walk test, the ataxia worsens with age and by 10 weeks the mutants also have splayed hindlegs determined by footprint analysis
Exp Paradigm: Accelerating rotarod test
|
Accelerating rotarod test |
6 weeks, 5 months, 2.5 months |
| |
|
Description: Mecp2-pvn ko mice have increased ataxia measured as shorter latency to fall of the accelerating rotarod and in the dowel walk test, the ataxia worsens with age and by 10 weeks the mutants also have splayed hindlegs determined by footprint analysis
Exp Paradigm: Accelerating rotarod test |
|
|
| Motor phenotype |
Motor coordination and balance1 |
Decreased
Description: Mecp2 pvn ko mice have reduced motor coordination and balance as the latency to fall from the accelerating rotarod and dowel walk assay, authors also attribute reduced marble burying to reduced coordination in forelimbs
Exp Paradigm: Dowel walk test
|
Dowel walk test |
6 weeks, 5 months, 2.5 months |
| |
|
Description: Mecp2 pvn ko mice have reduced motor coordination and balance as the latency to fall from the accelerating rotarod and dowel walk assay, authors also attribute reduced marble burying to reduced coordination in forelimbs
Exp Paradigm: Dowel walk test |
|
|
| Repetitive behavior |
Repetitive digging1 |
Decreased
Description: Mecp2-pvn ko mice have decreased number of marbles buried in the marble burying task, authors attribute that to reduced coordination in the forelimbs
Exp Paradigm: NA
|
Marble-burying test |
5.2 months |
| |
|
Description: Mecp2-pvn ko mice have decreased number of marbles buried in the marble burying task, authors attribute that to reduced coordination in the forelimbs
Exp Paradigm: NA |
|
|
| Sensory |
Startle response: acoustic stimulus1 |
Decreased
Description: Mecp2-pvn ko mice have decreased response to startle response: acoustic stimulus
Exp Paradigm: NA
|
Startle response test |
8 weeks |
| |
|
Description: Mecp2-pvn ko mice have decreased response to startle response: acoustic stimulus
Exp Paradigm: NA |
|
|
| Social behavior |
Social interaction1 |
Abnormal
Description: In the partition test mecp2-pvn ko mice show increased interaction with stranger conspecific mouse in the second phase, after habituation with a familiar mouse and prior to reintroduction of the familiar mouse
Exp Paradigm: The test is conducted in three phases: first is interaction with a familiar mouse housed in on the other side of a partition in home cage, second is interaction with a novel conspecific, age-matched mouse, third is reintroduction of the first familiar mouse
|
Partition test |
3.5 months |
| |
|
Description: In the partition test mecp2-pvn ko mice show increased interaction with stranger conspecific mouse in the second phase, after habituation with a familiar mouse and prior to reintroduction of the familiar mouse
Exp Paradigm: The test is conducted in three phases: first is interaction with a familiar mouse housed in on the other side of a partition in home cage, second is interaction with a novel conspecific, age-matched mouse, third is reintroduction of the first familiar mouse |
|
|
| Developmental profile |
Mortality/lethality1 |
Increased
Description: Mecp2- pvn ko mice have increased mortality and do not survive beyond 29 - 30 weeks of age
Exp Paradigm: NA
|
General observations |
7.5 months |
| |
|
Description: Mecp2- pvn ko mice have increased mortality and do not survive beyond 29 - 30 weeks of age
Exp Paradigm: NA |
|
|
| Learning & memory |
Cued or contextual fear conditioning: memory of cue1 |
Decreased
Description: Mecp2-pvn ko mice have significantly reduced memory of cue, assessed by differences in freezing response, in the fear conditioning paradigm compared to control floxed mecp2 mice that is significant at 15 weeks of age
Exp Paradigm: NA
|
Fear conditioning test |
3.7 months |
| |
|
Description: Mecp2-pvn ko mice have significantly reduced memory of cue, assessed by differences in freezing response, in the fear conditioning paradigm compared to control floxed mecp2 mice that is significant at 15 weeks of age
Exp Paradigm: NA |
|
|
| Molecular profile |
Targeted expression1 |
Decreased
Description: Mecp2 protein is ablated in the parvalbumin (pv) expressing interneurons
Exp Paradigm: NA
|
Immunofluorescence staining |
NA |
| |
|
Description: Mecp2 protein is ablated in the parvalbumin (pv) expressing interneurons
Exp Paradigm: NA |
|
|
| Developmental profile |
Size/growth1 |
No change |
Body weight measurement |
1-6 months |
| Emotion |
Anxiety1 |
No change |
Elevated plus maze test |
5 weeks |
| Emotion |
Anxiety1 |
No change |
Light-dark exploration test |
5 weeks |
| Motor phenotype |
General locomotor activity1 |
No change |
Open field test |
4.75 months |
| Repetitive behavior |
Repetitive nose pokes1 |
No change |
Hole-board test |
3 months |
| Seizure |
Seizures1 |
No change |
Observation of seizures |
3.7 - 10 months |
| Sensory |
Sensorimotor gating1 |
No change |
Prepulse inhibition |
8 weeks |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Immune response, Maternal behavior, Neuroanatomy / ultrastructure / cytoarchitecture, Neurophysiology, Physiological parameters, |
