| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
Motor coordination and balance1 |
Decreased
Description: Decreased motoro coordination indicated by greater number of footslips
Exp Paradigm: Grid walking test
|
Footslip test |
4 months |
| |
|
Description: Decreased motoro coordination indicated by greater number of footslips
Exp Paradigm: Grid walking test |
|
|
| Motor phenotype |
General locomotor activity1 |
Decreased
Description: Decreased locomotor activity indicated by less distance traveled and reduced horizontal and vertical activity
Exp Paradigm: Open field test
|
Open field test |
4 months |
| |
|
Description: Decreased locomotor activity indicated by less distance traveled and reduced horizontal and vertical activity
Exp Paradigm: Open field test |
|
|
| Sensory |
Startle response: acoustic stimulus1 |
Decreased
Description: Decreased startle response: acoustic stimulus response to 70-102 db, 102 to 118 db, and max 120 db audio stimulus
Exp Paradigm: Sensorimotor gating response to series of sounds from 70 to 118 db
|
Startle response test |
7, 20 weeks |
| |
|
Description: Decreased startle response: acoustic stimulus response to 70-102 db, 102 to 118 db, and max 120 db audio stimulus
Exp Paradigm: Sensorimotor gating response to series of sounds from 70 to 118 db |
|
|
| Sensory |
Sensorimotor gating1 |
Increased
Description: Increased prepulse inhibition (ppi) of the startle response: acoustic stimulus
Exp Paradigm: Prepulse inhibition of sensorimotor gating
|
Prepulse inhibition |
7, 20 weeks |
| |
|
Description: Increased prepulse inhibition (ppi) of the startle response: acoustic stimulus
Exp Paradigm: Prepulse inhibition of sensorimotor gating |
|
|
| Sensory |
Pain or nociception1 |
Decreased
Description: Decreased pain sensitivity to conductive heat stimulus indicated by increase in latency to respond when placed on a heted metal surface
Exp Paradigm: Female mice: hot plate test
|
Hot plate test |
5 months |
| |
|
Description: Decreased pain sensitivity to conductive heat stimulus indicated by increase in latency to respond when placed on a heted metal surface
Exp Paradigm: Female mice: hot plate test |
|
|
| Social behavior |
Social approach1 |
Decreased
Description: Decreased social approach indicated by less time spent investigating novel mouse
Exp Paradigm: Three chambered assay for sociability
|
Three-chamber social approach test |
5 months |
| |
|
Description: Decreased social approach indicated by less time spent investigating novel mouse
Exp Paradigm: Three chambered assay for sociability |
|
|
| Physiological parameters |
Respiratory function1 |
Abnormal
Description: Abnormal respiratory function indicative of breathing abnormalities through number of apneas per 1000 breaths
Exp Paradigm: Number of apneas and respiratory rate
|
Respiration rate and pattern analysis |
7 weeks |
| |
|
Description: Abnormal respiratory function indicative of breathing abnormalities through number of apneas per 1000 breaths
Exp Paradigm: Number of apneas and respiratory rate |
|
|
| Developmental profile |
Size/growth1 |
Increased
Description: Increased body weight
Exp Paradigm: General observations
|
General observations |
8-22 weeks |
| |
|
Description: Increased body weight
Exp Paradigm: General observations |
|
|
| Emotion |
Anxiety1 |
Decreased
Description: Decreased anxiety indicated by more time in open arms along with more open arm entries and more distance traveled
Exp Paradigm: Elevated plus maze test
|
Elevated plus maze test |
5 weeks |
| |
|
Description: Decreased anxiety indicated by more time in open arms along with more open arm entries and more distance traveled
Exp Paradigm: Elevated plus maze test |
|
|
| Emotion |
Anxiety1 |
Decreased
Description: Decreased anxiety indicated by more time spent in lit compartment
Exp Paradigm: Light/dark box assay
|
Light-dark exploration test |
5 weeks |
| |
|
Description: Decreased anxiety indicated by more time spent in lit compartment
Exp Paradigm: Light/dark box assay |
|
|
| Learning & memory |
Cued or contextual fear conditioning: passive avoidance1 |
Decreased
Description: Decreased passive avoidance learning indicated by decreased latency to enter dark compartment and fewer animals associating shock with dark compartment
Exp Paradigm: Passive avoidance task
|
Passive avoidance test |
14 weeks |
| |
|
Description: Decreased passive avoidance learning indicated by decreased latency to enter dark compartment and fewer animals associating shock with dark compartment
Exp Paradigm: Passive avoidance task |
|
|
| Learning & memory |
Cued or contextual fear conditioning: memory of context1 |
Decreased
Description: Decreased contextual fear memory but normal cued fear memory
Exp Paradigm: Pavlovian fear conditioning
|
Fear conditioning test |
14 weeks |
| |
|
Description: Decreased contextual fear memory but normal cued fear memory
Exp Paradigm: Pavlovian fear conditioning |
|
|
| Molecular profile |
Protein expression level evidence1 |
Decreased
Description: Decreased expression of mecp2 in hypothalamus to 40 to 85% levels
Exp Paradigm: Mecp2 levels
|
Quantitative pcr (qrt-pcr) |
5 months |
| |
|
Description: Decreased expression of mecp2 in hypothalamus to 40 to 85% levels
Exp Paradigm: Mecp2 levels |
|
|
| Motor phenotype |
General locomotor activity1 |
No change |
Open field test |
7 weeks |
| Sensory |
Olfaction1 |
No change |
Olfactory discrimination test |
12 weeks |
| Social behavior |
Social approach1 |
No change |
Three-chamber social approach test |
12 weeks |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Immune response, Maternal behavior, Neuroanatomy / ultrastructure / cytoarchitecture, Neurophysiology, Physiological parameters, Repetitive behavior, Seizure, |
