| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
General locomotor activity1 |
Decreased
Description: Avl-cre mecp2 have reduced general locomotor activity in open field
Exp Paradigm: NA
|
Open field test |
6-8 weeks |
| |
|
Description: Avl-cre mecp2 have reduced general locomotor activity in open field
Exp Paradigm: NA |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Synaptic neuroreceptors1 |
Decreased
Description: Avl-cre mecp2 spinal cords have reduced expression of gabrb3 in presynaptic puncta that are vglut +ve, observed in the spinal cord dorsal horn lamina iii
Exp Paradigm: Males only
|
Immunohistochemistry |
3-6 months |
| |
|
Description: Avl-cre mecp2 spinal cords have reduced expression of gabrb3 in presynaptic puncta that are vglut +ve, observed in the spinal cord dorsal horn lamina iii
Exp Paradigm: Males only |
|
|
| Neurophysiology |
Dorsal root potential (drp)1 |
Decreased
Description: Avl-cre mecp2 have decreased dorsal root potential indicating decrease in activity dependent presynaptic inhibition (psi) in the dorsal root, authors indicate that loss of mecp2 from primary somatosensory neurons causes deficiency of gabaa receptors on somatosensory neurons terminals that are known to mediate psi (and psi is decreased) this in turn causes increase in sensitivity to light touch
Exp Paradigm: NA
|
Whole-cell patch clamp |
P14-p19 |
| |
|
Description: Avl-cre mecp2 have decreased dorsal root potential indicating decrease in activity dependent presynaptic inhibition (psi) in the dorsal root, authors indicate that loss of mecp2 from primary somatosensory neurons causes deficiency of gabaa receptors on somatosensory neurons terminals that are known to mediate psi (and psi is decreased) this in turn causes increase in sensitivity to light touch
Exp Paradigm: NA |
|
|
| Neurophysiology |
Quantal post synaptic current frequency: excitatory1 |
Increased
Description: Psdcs have increased frequency of evoked qepscs in avl-cre mecp2 mice indicating changes in presynaptic release probability
Exp Paradigm: NA
|
Whole-cell patch clamp |
P14-p19 |
| |
|
Description: Psdcs have increased frequency of evoked qepscs in avl-cre mecp2 mice indicating changes in presynaptic release probability
Exp Paradigm: NA |
|
|
| Neurophysiology |
Neurotransmitter release: quantal parameters1 |
Increased
Description: Avl-cre mecp2 mechanosensory afferents that synapse to postsynaptic dorsal column projection neurons may have enhanced probability of presynaptic release of neurotransmitter, indirectly measured using quantal epsc frequency and decay times of currents
Exp Paradigm: NA
|
Whole-cell patch clamp |
6-8 weeks |
| |
|
Description: Avl-cre mecp2 mechanosensory afferents that synapse to postsynaptic dorsal column projection neurons may have enhanced probability of presynaptic release of neurotransmitter, indirectly measured using quantal epsc frequency and decay times of currents
Exp Paradigm: NA |
|
|
| Sensory |
Sensorimotor gating: tactile cue1 |
Decreased
Description: Avl-cre mecp2 decreased sensorimotor gating resulting in enhanced response in hairy skin tactile cue based prepulse inhibition compared to controls
Exp Paradigm: NA
|
Prepulse inhibition |
6-8 weeks |
| |
|
Description: Avl-cre mecp2 decreased sensorimotor gating resulting in enhanced response in hairy skin tactile cue based prepulse inhibition compared to controls
Exp Paradigm: NA |
|
|
| Sensory |
Touch1 |
Increased
Description: Avl-cre mecp2 have increased sensitivity towards touch or air puff on hairy skin
Exp Paradigm: NA
|
Response to air puff |
6-8 weeks |
| |
|
Description: Avl-cre mecp2 have increased sensitivity towards touch or air puff on hairy skin
Exp Paradigm: NA |
|
|
| Sensory |
Tactile memory1 |
Decreased
Description: Avl-cre mecp2 have decreased glabrous skin tactile discrimination and cannot differentiate between novel or familiar object differing in texture
Exp Paradigm: NA
|
Textured novel object recognition test (t-nort) |
6-8 weeks |
| |
|
Description: Avl-cre mecp2 have decreased glabrous skin tactile discrimination and cannot differentiate between novel or familiar object differing in texture
Exp Paradigm: NA |
|
|
| Social behavior |
Social approach1 |
Decreased
Description: Avl-cre mecp2 have decreased preference for stranger mouse over object compared to controls
Exp Paradigm: Males only
|
Three-chamber social approach test |
6-8 weeks |
| |
|
Description: Avl-cre mecp2 have decreased preference for stranger mouse over object compared to controls
Exp Paradigm: Males only |
|
|
| Social behavior |
Nest building behavior1 |
Decreased
Description: Avl-cre mecp2 have reduced nest building ability as more nest building material is left intact
Exp Paradigm: Males only
|
Nest building assay |
6-8 weeks |
| |
|
Description: Avl-cre mecp2 have reduced nest building ability as more nest building material is left intact
Exp Paradigm: Males only |
|
|
| Social behavior |
Social memory1 |
Decreased
Description: Avl-cre mecp2 have decreased preference for novel mouse over familiar mouse compared to controls
Exp Paradigm: Males only
|
Three-chamber social approach test |
6-8 weeks |
| |
|
Description: Avl-cre mecp2 have decreased preference for novel mouse over familiar mouse compared to controls
Exp Paradigm: Males only |
|
|
| Social behavior |
Social dominance1 |
Decreased
Description: Avl-cre mecp2 have significantly reduced wins compared to controls in tube test of dominance
Exp Paradigm: Males only
|
Tube test of social dominance |
6-8 weeks |
| |
|
Description: Avl-cre mecp2 have significantly reduced wins compared to controls in tube test of dominance
Exp Paradigm: Males only |
|
|
| Emotion |
Anxiety1 |
Increased
Description: Avl-cre mecp2 have reduced time spent in the center of the open field and reduced preference for open arms compared to controls indicating increase in anxiety
Exp Paradigm: Open field test
|
Open field test |
6-8 weeks |
| |
|
Description: Avl-cre mecp2 have reduced time spent in the center of the open field and reduced preference for open arms compared to controls indicating increase in anxiety
Exp Paradigm: Open field test |
|
|
| Emotion |
Anxiety1 |
Increased
Description: Avl-cre mecp2 have reduced time spent in the center of the open field and reduced preference for open arms compared to controls indicating increase in anxiety
Exp Paradigm: Elevated plus maze test
|
Elevated plus maze test |
6-8 weeks |
| |
|
Description: Avl-cre mecp2 have reduced time spent in the center of the open field and reduced preference for open arms compared to controls indicating increase in anxiety
Exp Paradigm: Elevated plus maze test |
|
|
| Emotion |
Habituation to aversive stimuli1 |
Decreased
Description: Avl-cre mecp2 have reduced habituation to aversive acoustic stimulus
Exp Paradigm: Males only
|
Startle response test |
6-8 weeks |
| |
|
Description: Avl-cre mecp2 have reduced habituation to aversive acoustic stimulus
Exp Paradigm: Males only |
|
|
| Developmental profile |
Mortality/lethality1 |
No change |
Survival analysis |
1-10 months |
| Developmental profile |
Size/growth1 |
No change |
Body weight measurement |
2-2.5 months |
| Emotion |
Exploratory activity1 |
No change |
Textured novel object recognition test (t-nort) |
6-8 weeks |
| Learning & memory |
Object recognition memory1 |
No change |
Novel object recognition test |
6-8 weeks |
| Motor phenotype |
Motor coordination and balance1 |
No change |
Accelerating rotarod test |
6-8 weeks |
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Brain size1 |
No change |
Histology |
3-6 months |
| Neurophysiology |
Decay kinetics of evoked post synaptic currents1 |
No change |
Whole-cell patch clamp |
P14-p19 |
| Neurophysiology |
Quantal post synaptic current amplitude: excitatory1 |
No change |
Whole-cell patch clamp |
P14-p19 |
| Neurophysiology |
Spinal reflex: ventral root potential (vrp)1 |
No change |
Whole-cell patch clamp |
P14-p19 |
| Physiological parameters |
Respiratory function1 |
No change |
Whole body plethysmography |
2-2.5 months |
| Sensory |
Sensorimotor gating1 |
No change |
Prepulse inhibition |
6-8 weeks |
| Sensory |
Startle response: acoustic stimulus1 |
No change |
Startle response test |
6-8 weeks |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Immune response, Maternal behavior, Molecular profile, Physiological parameters, Repetitive behavior, Seizure, |
