| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
Motor coordination and balance1 |
Decreased
Description: Mecp2 stop mice have reduced latency to fall off compared to controls in the accelerating rotarod
Exp Paradigm: NA
|
Accelerating rotarod test |
6-8 weeks |
| |
|
Description: Mecp2 stop mice have reduced latency to fall off compared to controls in the accelerating rotarod
Exp Paradigm: NA |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Brain size1 |
Decreased
Description: Mecp2 stop mice have decreased brain size compared to controls
Exp Paradigm: NA
|
Histology |
3-6 months |
| |
|
Description: Mecp2 stop mice have decreased brain size compared to controls
Exp Paradigm: NA |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Synaptic neuroreceptors1 |
Decreased
Description: Mecp2 stop mouse spinal cords have reduced expression of gabrb3 in presynaptic puncta that are vglut +ve, observed in the spinal cord dorsal horn lamina iii
Exp Paradigm: NA
|
Immunohistochemistry |
3-6 months |
| |
|
Description: Mecp2 stop mouse spinal cords have reduced expression of gabrb3 in presynaptic puncta that are vglut +ve, observed in the spinal cord dorsal horn lamina iii
Exp Paradigm: NA |
|
|
| Neurophysiology |
Dorsal root potential (drp)1 |
Decreased
Description: Dorsal root potential (drp) (peak) amplitude is significantly less than controls in mecp2 stop mice indicating reduced gabaa mediated presynaptic inhibition of somatosensory input into the dorsal horn (which may cause hyperexcitability due to lack of reduction of response mediated by cns to repeated tactile stimulation)
Exp Paradigm: NA
|
Whole-cell patch clamp |
P14-p19 |
| |
|
Description: Dorsal root potential (drp) (peak) amplitude is significantly less than controls in mecp2 stop mice indicating reduced gabaa mediated presynaptic inhibition of somatosensory input into the dorsal horn (which may cause hyperexcitability due to lack of reduction of response mediated by cns to repeated tactile stimulation)
Exp Paradigm: NA |
|
|
| Sensory |
Touch1 |
Increased
Description: Mecp2 stop mice have increased sensitivity to touch on hair skin by air puff
Exp Paradigm: NA
|
Response to air puff |
6-8 weeks |
| |
|
Description: Mecp2 stop mice have increased sensitivity to touch on hair skin by air puff
Exp Paradigm: NA |
|
|
| Sensory |
Sensorimotor gating1 |
Decreased
Description: Mecp2 stop mice have impaired sensorimotor gating when the prepulse is acoustic as well
Exp Paradigm: NA
|
Prepulse inhibition |
6-8 weeks |
| |
|
Description: Mecp2 stop mice have impaired sensorimotor gating when the prepulse is acoustic as well
Exp Paradigm: NA |
|
|
| Sensory |
Tactile memory1 |
Decreased
Description: Mecp2 stop mice have reduced tactile discrimination (glabrous skin) and memory for objects differing in texture (rough or smooth) and do not preferentially interact with a novel textured-object over a familiar one
Exp Paradigm: NA
|
Textured novel object recognition test (t-nort) |
6-8 weeks |
| |
|
Description: Mecp2 stop mice have reduced tactile discrimination (glabrous skin) and memory for objects differing in texture (rough or smooth) and do not preferentially interact with a novel textured-object over a familiar one
Exp Paradigm: NA |
|
|
| Sensory |
Startle response: acoustic stimulus1 |
Decreased
Description: Similar to other mecp2 null mice, mecp2 stop mice also have reduced response to acoustic startle at 125 db
Exp Paradigm: NA
|
Startle response test |
6-8 weeks |
| |
|
Description: Similar to other mecp2 null mice, mecp2 stop mice also have reduced response to acoustic startle at 125 db
Exp Paradigm: NA |
|
|
| Sensory |
Sensorimotor gating: tactile cue1 |
Decreased
Description: Mecp2 stop mice have impaired sensorimotor gating as they exhibit enhanced response to tactile prepulse (air puff)
Exp Paradigm: NA
|
Prepulse inhibition |
6-8 weeks |
| |
|
Description: Mecp2 stop mice have impaired sensorimotor gating as they exhibit enhanced response to tactile prepulse (air puff)
Exp Paradigm: NA |
|
|
| Social behavior |
Social dominance1 |
Decreased
Description: Mecp2 stop mice have reduced dominant or aggressive behavior as in the tube test of dominance they have significantly fewer wins compared to controls
Exp Paradigm: NA
|
Tube test of social dominance |
6-8 weeks |
| |
|
Description: Mecp2 stop mice have reduced dominant or aggressive behavior as in the tube test of dominance they have significantly fewer wins compared to controls
Exp Paradigm: NA |
|
|
| Social behavior |
Social approach1 |
Decreased
Description: Mecp2 stop mice have reduced preference for a stranger mouse over empty cage
Exp Paradigm: NA
|
Three-chamber social approach test |
6-8 weeks |
| |
|
Description: Mecp2 stop mice have reduced preference for a stranger mouse over empty cage
Exp Paradigm: NA |
|
|
| Social behavior |
Nest building behavior1 |
Decreased
Description: Mecp2 stop mice have reduced nest building material broken down than controls
Exp Paradigm: NA
|
Nest building assay |
6-8 weeks |
| |
|
Description: Mecp2 stop mice have reduced nest building material broken down than controls
Exp Paradigm: NA |
|
|
| Social behavior |
Social memory1 |
Decreased
Description: Mecp2 stop mice have reduced preference for a stranger mouse over familiar mouse
Exp Paradigm: NA
|
Three-chamber social approach test |
6-8 weeks |
| |
|
Description: Mecp2 stop mice have reduced preference for a stranger mouse over familiar mouse
Exp Paradigm: NA |
|
|
| Emotion |
Anxiety1 |
Increased
Description: Mecp2 stop mice show increased anxiety- like behavior in the open field (time spent in center) and elevated plus maze test (preference for open arms)
Exp Paradigm: Open field test
|
Open field test |
6-8 weeks |
| |
|
Description: Mecp2 stop mice show increased anxiety- like behavior in the open field (time spent in center) and elevated plus maze test (preference for open arms)
Exp Paradigm: Open field test |
|
|
| Emotion |
Anxiety1 |
Increased
Description: Mecp2 stop mice show increased anxiety- like behavior in the open field (time spent in center) and elevated plus maze test (preference for open arms)
Exp Paradigm: Elevated plus maze test
|
Elevated plus maze test |
6-8 weeks |
| |
|
Description: Mecp2 stop mice show increased anxiety- like behavior in the open field (time spent in center) and elevated plus maze test (preference for open arms)
Exp Paradigm: Elevated plus maze test |
|
|
| Emotion |
Habituation to aversive stimuli1 |
Decreased
Description: Mecp2 stop mice have reduced habituation to aversive startle noise compared to controls
Exp Paradigm: NA
|
Startle response test |
6-8 weeks |
| |
|
Description: Mecp2 stop mice have reduced habituation to aversive startle noise compared to controls
Exp Paradigm: NA |
|
|
| Learning & memory |
Object recognition memory: long-term recall1 |
Decreased
Description: Mecp2 stop mice have reduced long term recall of familiar objects
Exp Paradigm: NA
|
Novel object recognition test |
6-8 weeks |
| |
|
Description: Mecp2 stop mice have reduced long term recall of familiar objects
Exp Paradigm: NA |
|
|
| Emotion |
Exploratory activity1 |
No change |
Textured novel object recognition test (t-nort) |
6-8 weeks |
| Learning & memory |
Object recognition memory1 |
No change |
Novel object recognition test |
6-8 weeks |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Immune response, Maternal behavior, Molecular profile, Physiological parameters, Repetitive behavior, Seizure, |
