| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
Motor coordination and balance1 |
Restored
Description: In the aso rescue model mice motor behavior was restored to the level of wt and significantly increased compared to the vehicle treated tg mice
Exp Paradigm: Measured latency to fall
|
Accelerating rotarod test |
Adult |
| |
|
Description: In the aso rescue model mice motor behavior was restored to the level of wt and significantly increased compared to the vehicle treated tg mice
Exp Paradigm: Measured latency to fall |
|
|
| Social behavior |
Social approach1 |
Restored
Description: In the aso rescue model mice time spent investigating a cup with a mouse was restored to the level of wt control mice and significantly increased compared to vehicle treated tg mice; in the aso rescue model mice time spent investigating a cup with an object was not changed compared to the level of wt control mice or tg mice; no left or right preference was observed in the habituation phase;
Exp Paradigm: Measured time spent investing object cup and mouse cup
|
Three-chamber social approach test |
Adult |
| |
|
Description: In the aso rescue model mice time spent investigating a cup with a mouse was restored to the level of wt control mice and significantly increased compared to vehicle treated tg mice; in the aso rescue model mice time spent investigating a cup with an object was not changed compared to the level of wt control mice or tg mice; no left or right preference was observed in the habituation phase;
Exp Paradigm: Measured time spent investing object cup and mouse cup |
|
|
| Emotion |
Exploratory activity1 |
Restored
Description: In aso5 treated mecp2 tg mice exploratory activity was restored to the level of wt and significantly increased compared to mecp2 tg mice
Exp Paradigm: Measured horizontal activity, time in center and distance traversed in the center
|
Open field test |
Adult |
| |
|
Description: In aso5 treated mecp2 tg mice exploratory activity was restored to the level of wt and significantly increased compared to mecp2 tg mice
Exp Paradigm: Measured horizontal activity, time in center and distance traversed in the center |
|
|
| Emotion |
Anxiety1 |
Restored
Description: In aso5 treated mecp2 tg mice anxiety was restored to the level of wt and significantly reduced compared to mecp2 tg mice
Exp Paradigm: Measured time spent and distance traversed in open arms of elevated plus maze; rearing;
|
Elevated plus maze test |
Adult |
| |
|
Description: In aso5 treated mecp2 tg mice anxiety was restored to the level of wt and significantly reduced compared to mecp2 tg mice
Exp Paradigm: Measured time spent and distance traversed in open arms of elevated plus maze; rearing; |
|
|
| Molecular profile |
Gene expression1 |
Refractory
Description: Mouse mecp2e2 mrna splice variant expression is not changed in aso5 rescue model compared to vehicle treated mecp2 overexpressing tg parent model
Exp Paradigm: Cortical samples;
|
Quantitative pcr (qrt-pcr) |
6 weeks |
| |
|
Description: Mouse mecp2e2 mrna splice variant expression is not changed in aso5 rescue model compared to vehicle treated mecp2 overexpressing tg parent model
Exp Paradigm: Cortical samples; |
|
|
| Molecular profile |
Gene expression1 |
Refractory
Description: Mouse mecp2e1 mrna splice variant expression is not changed in aso5 rescue model compared to vehicle treated mecp2 overexpressing tg parent model
Exp Paradigm: Cortical samples;
|
Quantitative pcr (qrt-pcr) |
6 weeks |
| |
|
Description: Mouse mecp2e1 mrna splice variant expression is not changed in aso5 rescue model compared to vehicle treated mecp2 overexpressing tg parent model
Exp Paradigm: Cortical samples; |
|
|
| Molecular profile |
Targeted expression1 |
Restored
Description: In the aso rescue model mecp2 protein expression is reduced in the cortical samples (wb) and in the hippocampus and striatum (if) compared to mecp2 over-expressing tg mutant model;
Exp Paradigm: Cortical samples;-immunohistochemistry
|
Immunohistochemistry |
14 weeks |
| |
|
Description: In the aso rescue model mecp2 protein expression is reduced in the cortical samples (wb) and in the hippocampus and striatum (if) compared to mecp2 over-expressing tg mutant model;
Exp Paradigm: Cortical samples;-immunohistochemistry |
|
|
| Molecular profile |
Gene expression1 |
Ameliorated
Description: Human mecp2e2 mrna splice variant expression is significantly reduced in aso5 rescue model compared to vehicle treated mecp2 overexpressing tg parent model
Exp Paradigm: Cortical samples;
|
Quantitative pcr (qrt-pcr) |
6 weeks |
| |
|
Description: Human mecp2e2 mrna splice variant expression is significantly reduced in aso5 rescue model compared to vehicle treated mecp2 overexpressing tg parent model
Exp Paradigm: Cortical samples; |
|
|
| Molecular profile |
Targeted expression1 |
Restored
Description: In the aso rescue model mecp2 protein expression is reduced in the cortical samples (wb) and in the hippocampus and striatum (if) compared to mecp2 over-expressing tg mutant model;
Exp Paradigm: Cortical samples;- western blot
|
Western blot |
14 weeks |
| |
|
Description: In the aso rescue model mecp2 protein expression is reduced in the cortical samples (wb) and in the hippocampus and striatum (if) compared to mecp2 over-expressing tg mutant model;
Exp Paradigm: Cortical samples;- western blot |
|
|
| Molecular profile |
Gene expression1 |
Ameliorated
Description: Human mecp2e1 mrna splice variant expression is significantly reduced in aso5 rescue model compared to vehicle treated mecp2 overexpressing tg parent model
Exp Paradigm: Cortical samples;
|
Quantitative pcr (qrt-pcr) |
6 weeks |
| |
|
Description: Human mecp2e1 mrna splice variant expression is significantly reduced in aso5 rescue model compared to vehicle treated mecp2 overexpressing tg parent model
Exp Paradigm: Cortical samples; |
|
|
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Immune response, Learning & memory, Maternal behavior, Neuroanatomy / ultrastructure / cytoarchitecture, Neurophysiology, Physiological parameters, Repetitive behavior, Seizure, Sensory, |
