| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
General locomotor activity1 |
Decreased
Description: Decreased locomotor activity
Exp Paradigm: Open field test
|
Open field test |
9, 11 weeks |
| |
|
Description: Decreased locomotor activity
Exp Paradigm: Open field test |
|
|
| Motor phenotype |
Motor coordination and balance1 |
Decreased
Description: Decreased motor coordination and motor learning
Exp Paradigm: Rotarod test
|
Accelerating rotarod test |
Unreported |
| |
|
Description: Decreased motor coordination and motor learning
Exp Paradigm: Rotarod test |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Brain size1 |
Decreased
Description: Decreased size and weight of brains
Exp Paradigm: NA
|
Measurement of tissue weight |
4,13 weeks |
| |
|
Description: Decreased size and weight of brains
Exp Paradigm: NA |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Brain cytoarchitecture1 |
Decreased
Description: Decreased soma size in hippocampal ca1 region
Exp Paradigm: Confocal imaging anlysis of gfp-positive pyramidal neurons
|
Confocal microscopy |
4,13 weeks |
| |
|
Description: Decreased soma size in hippocampal ca1 region
Exp Paradigm: Confocal imaging anlysis of gfp-positive pyramidal neurons |
|
|
| Neurophysiology |
Event related potential (erp) in electroencephalography (eeg)1 |
Increased
Description: Increased latency of initial positive peak, negative peak and a subsequent second positive peak
Exp Paradigm: Eeg recordings following presentatino of a series of white noise clicks
|
Electroencephalogram (eeg) |
3 months |
| |
|
Description: Increased latency of initial positive peak, negative peak and a subsequent second positive peak
Exp Paradigm: Eeg recordings following presentatino of a series of white noise clicks |
|
|
| Neurophysiology |
Event related potential (erp) in electroencephalography (eeg)1 |
Decreased
Description: Decreased reduction in event related power in low frequency gamma, theta, and alpha
Exp Paradigm: Time frequency analysis on eeg recordings
|
Electroencephalogram (eeg) |
4 weeks |
| |
|
Description: Decreased reduction in event related power in low frequency gamma, theta, and alpha
Exp Paradigm: Time frequency analysis on eeg recordings |
|
|
| Neurophysiology |
Event related potential (erp) in electroencephalography (eeg)1 |
Decreased
Description: Decreased event-related power in both low and high frequency oscillations
Exp Paradigm: Time frequency analysis on eeg recordings
|
Electroencephalogram (eeg) |
13 weeks |
| |
|
Description: Decreased event-related power in both low and high frequency oscillations
Exp Paradigm: Time frequency analysis on eeg recordings |
|
|
| Neurophysiology |
Event related potential (erp) in electroencephalography (eeg)1 |
Abnormal
Description: Abnormal eeg recordings indicated by increased power of high-gamma frequency oscillations
Exp Paradigm: Eeg recordings in awake, freely mobile
|
Electroencephalogram (eeg) |
3 months |
| |
|
Description: Abnormal eeg recordings indicated by increased power of high-gamma frequency oscillations
Exp Paradigm: Eeg recordings in awake, freely mobile |
|
|
| Developmental profile |
Mortality/lethality1 |
Increased
Description: Increased lethality
Exp Paradigm: Male mice: general observation
|
General observations |
16 weeks |
| |
|
Description: Increased lethality
Exp Paradigm: Male mice: general observation |
|
|
| Developmental profile |
Developmental trajectory1 |
Abnormal
Description: Abnormal developmental trajectory with progressive symptomology
Exp Paradigm: Male mice: general observation
|
General observations |
5 weeks |
| |
|
Description: Abnormal developmental trajectory with progressive symptomology
Exp Paradigm: Male mice: general observation |
|
|
| Developmental profile |
Size/growth1 |
Decreased
Description: Decreased body weight
Exp Paradigm: Male mice: general observation
|
General observations |
4 weeks |
| |
|
Description: Decreased body weight
Exp Paradigm: Male mice: general observation |
|
|
| Emotion |
Anxiety1 |
Decreased
Description: Decreased anxiety indicated by more time spent in open vs. closed arm
Exp Paradigm: Elevated zero maze test
|
Elevated zero maze test |
9 weeks |
| |
|
Description: Decreased anxiety indicated by more time spent in open vs. closed arm
Exp Paradigm: Elevated zero maze test |
|
|
| Learning & memory |
Cued or contextual fear conditioning1 |
Decreased
Description: Decreased learning and memory indicated by decreased context-dependent and cue-dependent freezing
Exp Paradigm: Contextual and cued fear-conditioning procedure
|
Fear conditioning test |
10 weeks |
| |
|
Description: Decreased learning and memory indicated by decreased context-dependent and cue-dependent freezing
Exp Paradigm: Contextual and cued fear-conditioning procedure |
|
|
| Molecular profile |
Gene expression1 |
Decreased
Description: Decreased transcription of bdnf and crh in hypothalamus but not striatum
Exp Paradigm: Transcriptional profiling
|
Quantitative pcr (qrt-pcr) |
Unreported |
| |
|
Description: Decreased transcription of bdnf and crh in hypothalamus but not striatum
Exp Paradigm: Transcriptional profiling |
|
|
| Molecular profile |
Protein-dna complex assembly1 |
Decreased
Description: Decreased binding of of mecp2 across the entire locus
Exp Paradigm: Mecp2 binding
|
Quantitative pcr (qrt-pcr) |
Unreported |
| |
|
Description: Decreased binding of of mecp2 across the entire locus
Exp Paradigm: Mecp2 binding |
|
|
| Molecular profile |
Targeted expression1 |
Decreased
Description: Decreased interaction with hdac1 and sin3a
Exp Paradigm: Mecp2 immunoreactivity-immunoprecipitation
|
Immunoprecipitation |
Unreported |
| |
|
Description: Decreased interaction with hdac1 and sin3a
Exp Paradigm: Mecp2 immunoreactivity-immunoprecipitation |
|
|
| Molecular profile |
Protein-dna complex assembly1 |
Decreased
Description: Decreased affinity of mecp2 to methylated dna indicated by more readily extraction under low salt conditions
Exp Paradigm: Mecp2 affinity to methylated dna
|
Western blot |
Unreported |
| Molecular profile |
Targeted expression1 |
Decreased
Description: Decreased interaction with hdac1 and sin3a
Exp Paradigm: Mecp2 immunoreactivity- western blot: anti-mecp2 antibody/anti-hdac1 or anti-sin3a antibody
|
Western blot |
Unreported |
| |
|
Description: Decreased interaction with hdac1 and sin3a
Exp Paradigm: Mecp2 immunoreactivity- western blot: anti-mecp2 antibody/anti-hdac1 or anti-sin3a antibody |
|
|
| Molecular profile |
Protein expression level evidence1 |
Decreased
Description: Decreased mecp2 protein expression
Exp Paradigm: Mecp2 protein expression
|
Western blot |
P2, 1, 3 months |
| |
|
Description: Decreased mecp2 protein expression
Exp Paradigm: Mecp2 protein expression |
|
|
| Molecular profile |
Targeted expression1 |
Abnormal
Description: Abnormally diffuse mecp2 immunoreactivity throughout nucleus with clear absence of co-localization with dna
Exp Paradigm: Mecp2 immunoreactivity
|
Confocal microscopy |
1 month, 3 months |
| |
|
Description: Abnormally diffuse mecp2 immunoreactivity throughout nucleus with clear absence of co-localization with dna
Exp Paradigm: Mecp2 immunoreactivity |
|
|
| Developmental profile |
General characteristics1 |
No change |
General observations |
4 weeks |
| Developmental profile |
Size/growth1 |
No change |
General observations |
8 weeks |
| Molecular profile |
Gene expression1 |
No change |
Quantitative pcr (qrt-pcr) |
P0, 1,3 months |
| Motor phenotype |
General locomotor activity1 |
No change |
Open field test |
3 weeks |
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Brain anatomy1 |
No change |
Histology |
Unreported |
| Neurophysiology |
Event related potential (erp) in electroencephalography (eeg)1 |
No change |
Electroencephalogram (eeg) |
4 weeks |
| Neurophysiology |
Event related potential (erp) in electroencephalography (eeg)1 |
No change |
Electroencephalogram (eeg) |
4 weeks |
| Sensory |
Hearing1 |
No change |
Auditory brainstem response test |
1-3 months |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Immune response, Maternal behavior, Physiological parameters, Repetitive behavior, Seizure, Social behavior, |
