| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
Grip strength1 |
Ameliorated
Description: Decreased grip strength is partially restored to the level of wild type on pn 22 mice; decreased grip strength is refractoryto the treatment on pn 30 or pn 43 mice
Exp Paradigm: Grip strength assay
|
Grip strength test |
3, 4, 6 weeks |
| |
|
Description: Decreased grip strength is partially restored to the level of wild type on pn 22 mice; decreased grip strength is refractoryto the treatment on pn 30 or pn 43 mice
Exp Paradigm: Grip strength assay |
|
|
| Motor phenotype |
Grip strength1 |
Ameliorated
Description: Decreased forepaw grip strength is partially restored to the level of wild type
Exp Paradigm: Grip strength assay
|
Grip strength test |
6 weeks |
| |
|
Description: Decreased forepaw grip strength is partially restored to the level of wild type
Exp Paradigm: Grip strength assay |
|
|
| Motor phenotype |
General locomotor activity1 |
Ameliorated
Description: Decreased locomotor activity is partially restored to the level of wild type on pn 21 or pn 29 mice but is refractoryto the treatment on pn 42 mice
Exp Paradigm: General motor activity levels during dark cycle
|
Light-dark exploration test |
3, 4, 6 weeks |
| |
|
Description: Decreased locomotor activity is partially restored to the level of wild type on pn 21 or pn 29 mice but is refractoryto the treatment on pn 42 mice
Exp Paradigm: General motor activity levels during dark cycle |
|
|
| Motor phenotype |
Motor coordination and balance1 |
Refractory
Description: Decreased motor coordination is refractoryto the treatment
Exp Paradigm: Rotorod test
|
Accelerating rotarod test |
6 weeks |
| |
|
Description: Decreased motor coordination is refractoryto the treatment
Exp Paradigm: Rotorod test |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Dendritic architecture: spine density1 |
Restored
Description: Dendritic cell morphology assessed by cell body size, dendritic length and dendritic structure is restored to the same level as wild type
Exp Paradigm: Sholl analysis of cell morphology in the dentate gyrus of the hippocampus
|
Sholl analysis |
6.5 weeks |
| |
|
Description: Dendritic cell morphology assessed by cell body size, dendritic length and dendritic structure is restored to the same level as wild type
Exp Paradigm: Sholl analysis of cell morphology in the dentate gyrus of the hippocampus |
|
|
| Neurophysiology |
Neurotransmitter release: neurotrophins1 |
Refractory
Description: Decreased bdnf expression in cerebellum or decreased ngf expression in striatum is refractoryto the treatment; there are no adverse effectin bdnf/ngf expresson in other checked brain areas (cortex, hippocampus, and striatum/cerebellum)
Exp Paradigm: Bdnf/ngf elisa's
|
Immunohistochemistry |
6 weeks |
| |
|
Description: Decreased bdnf expression in cerebellum or decreased ngf expression in striatum is refractoryto the treatment; there are no adverse effectin bdnf/ngf expresson in other checked brain areas (cortex, hippocampus, and striatum/cerebellum)
Exp Paradigm: Bdnf/ngf elisa's |
|
|
| Physiological parameters |
Metabolic function1 |
Restored
Description: Metabolic function as assessed by measuring carbon dioxide (co2) production and oxygen (o2) consumption is restored to the same level as wild type on pn 43 mice; there is no adverse effectin pn 22 or pn 30 mice
Exp Paradigm: Gas exchange measurements
|
Measurement of gas exchange |
3, 4, 6 weeks |
| |
|
Description: Metabolic function as assessed by measuring carbon dioxide (co2) production and oxygen (o2) consumption is restored to the same level as wild type on pn 43 mice; there is no adverse effectin pn 22 or pn 30 mice
Exp Paradigm: Gas exchange measurements |
|
|
| Physiological parameters |
Bioactive compound levels: carnitine1 |
Side effect
Description: Increased levels of l-carnitine, alc, and propionyl carnitine (plc) in blood plasma is a direct effect after the treatment
Exp Paradigm: Carnitines levels
|
High-performance liquid chromatography (hplc) |
6.5 weeks |
| |
|
Description: Increased levels of l-carnitine, alc, and propionyl carnitine (plc) in blood plasma is a direct effect after the treatment
Exp Paradigm: Carnitines levels |
|
|
| Developmental profile |
Size/growth1 |
Ameliorated
Description: Decreased rate of weight gain is restored to the same level as wild type during pn 22-35 but is refractoryto the treatment after pn 36
Exp Paradigm: General observations
|
General observations |
3-5 weeks |
| |
|
Description: Decreased rate of weight gain is restored to the same level as wild type during pn 22-35 but is refractoryto the treatment after pn 36
Exp Paradigm: General observations |
|
|
| Learning & memory |
Cued or contextual fear conditioning1 |
Refractory
Description: Decreased in cued conditioning learning is refractoryto the treatment but there is no adverse effectin contextual conditioning learning
Exp Paradigm: Associative fear conditioning task
|
Fear conditioning test |
6 weeks |
| |
|
Description: Decreased in cued conditioning learning is refractoryto the treatment but there is no adverse effectin contextual conditioning learning
Exp Paradigm: Associative fear conditioning task |
|
|
| Learning & memory |
Object recognition memory1 |
Ameliorated
Description: Decreased long term memory is partially restored to the level of wild type
Exp Paradigm: Object recognition task
|
Novel object recognition test |
4 weeks |
| |
|
Description: Decreased long term memory is partially restored to the level of wild type
Exp Paradigm: Object recognition task |
|
|
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Emotion, Immune response, Maternal behavior, Physiological parameters, Repetitive behavior, Seizure, Sensory, Social behavior, |
