| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Neurophysiology |
Synaptic transmission2 |
Increased
Description: Increased amplitude and frequency of miniature epsc's
Exp Paradigm: Electrophysiological recordings of epsc evoked by stimulation of ts at 20 hz with ttx and bicuuciline application
|
Whole-cell patch clamp |
5-7 weeks |
| |
|
Description: Increased amplitude and frequency of miniature epsc's
Exp Paradigm: Electrophysiological recordings of epsc evoked by stimulation of ts at 20 hz with ttx and bicuuciline application |
|
|
| Neurophysiology |
Miniature post synaptic current amplitude: excitatory2 |
Abnormal
Description: Abnormal synaptic function demonstrated by increased ts-epsc amplitude
Exp Paradigm: Epsc recordings after stimulation of fibers of horizontal brain stem slices
|
Whole-cell patch clamp |
5-7 weeks |
| |
|
Description: Abnormal synaptic function demonstrated by increased ts-epsc amplitude
Exp Paradigm: Epsc recordings after stimulation of fibers of horizontal brain stem slices |
|
|
| Neurophysiology |
Action potential property: firing rate2 |
Increased
Description: Increased action potential firing probability
Exp Paradigm: Epsc recordings in response to 20 hz stimulation of nts neurons in horizontal brain slices
|
Whole-cell patch clamp |
5-7 weeks |
| |
|
Description: Increased action potential firing probability
Exp Paradigm: Epsc recordings in response to 20 hz stimulation of nts neurons in horizontal brain slices |
|
|
| Physiological parameters |
Respiratory function1 |
Increased
Description: The mean respiratory frequency is very high in mecp2 null mice compared to wt controls , arising of repetitive episodes of very high breathing frequency. therefore the minute volume/ weight is increased in mecp2 null mcie
Exp Paradigm: Tidal volume/ weight and minute volume/weight (tidal volume/weight x breathing frequency) were estimated
|
Whole body plethysmography |
5 weeks |
| |
|
Description: The mean respiratory frequency is very high in mecp2 null mice compared to wt controls , arising of repetitive episodes of very high breathing frequency. therefore the minute volume/ weight is increased in mecp2 null mcie
Exp Paradigm: Tidal volume/ weight and minute volume/weight (tidal volume/weight x breathing frequency) were estimated |
|
|
| Physiological parameters |
Respiratory function1 |
Abnormal
Description: Mecp2 null mice have a disordered breathing pattern characterized by a highly variable frequency and occasional long breathing pauses compared to wt littermates.
Exp Paradigm: NA
|
Whole body plethysmography |
5 weeks |
| |
|
Description: Mecp2 null mice have a disordered breathing pattern characterized by a highly variable frequency and occasional long breathing pauses compared to wt littermates.
Exp Paradigm: NA |
|
|
| Molecular profile |
Protein expression level evidence1 |
Decreased
Description: Bdnf levels in vehicle injected mecp2 null mice was significantly reduced in the nodose cranial sensory ganglia neurons , compared to vehicle injected wild type controls
Exp Paradigm: Protein expression
|
Elisa |
5 weeks |
| |
|
Description: Bdnf levels in vehicle injected mecp2 null mice was significantly reduced in the nodose cranial sensory ganglia neurons , compared to vehicle injected wild type controls
Exp Paradigm: Protein expression |
|
|
| Molecular profile |
Gene expression1 |
Decreased
Description: Mrna levels of brain derived neurotrophic factor (bdnf) containing exons 2, 4 or 5 are reduced in the neurons from the nodose cranial sensory ganglia (ngs) derived from mecp2 null mice
Exp Paradigm: Gene expression
|
Quantitative pcr (qrt-pcr) |
5 weeks |
| |
|
Description: Mrna levels of brain derived neurotrophic factor (bdnf) containing exons 2, 4 or 5 are reduced in the neurons from the nodose cranial sensory ganglia (ngs) derived from mecp2 null mice
Exp Paradigm: Gene expression |
|
|
| Molecular profile |
Protein expression level evidence2 |
Decreased
Description: Decreased bndf staining in perikarya and fibers of dorsal motor nucleus of vagus nerve, nts, area postrema, nucleus ambiguus, medullary reticular nuclei
Exp Paradigm: Bdnf protein expression
|
Immunohistochemistry |
5 weeks |
| |
|
Description: Decreased bndf staining in perikarya and fibers of dorsal motor nucleus of vagus nerve, nts, area postrema, nucleus ambiguus, medullary reticular nuclei
Exp Paradigm: Bdnf protein expression |
|
|
| Neurophysiology |
Action potential property: after hyperpolarization2 |
No change |
Whole-cell patch clamp |
5-7 weeks |
| Neurophysiology |
Action potential property: amplitude2 |
No change |
Whole-cell patch clamp |
5-7 weeks |
| Neurophysiology |
Action potential property: firing rate2 |
No change |
Whole-cell patch clamp |
5-7 weeks |
| Neurophysiology |
Action potential property: half-width2 |
No change |
Whole-cell patch clamp |
5-7 weeks |
| Neurophysiology |
Action potential property: threshold2 |
No change |
Whole-cell patch clamp |
5-7 weeks |
| Neurophysiology |
Intrinsic membrane properties2 |
No change |
Whole-cell patch clamp |
5-7 weeks |
| Neurophysiology |
Membrane potential2 |
No change |
Whole-cell patch clamp |
5-7 weeks |
| Neurophysiology |
Synaptic transmission: inhibitory2 |
No change |
Whole-cell patch clamp |
5-7 weeks |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Emotion, Immune response, Learning & memory, Maternal behavior, Motor phenotype, Neuroanatomy / ultrastructure / cytoarchitecture, Neurophysiology, Physiological parameters, Repetitive behavior, Seizure, Sensory, Social behavior, |
