| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Neurophysiology |
Miniature post synaptic current frequency: excitatory1 |
Ameliorated
Description: Ameliorated frequency of mepscs compared to parent model, not significantly higher than wild type
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla)
|
Whole-cell patch clamp |
2-3 months |
| |
|
Description: Ameliorated frequency of mepscs compared to parent model, not significantly higher than wild type
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla) |
|
|
| Neurophysiology |
Spontaneous post synaptic events: inhibitory currents1 |
Restored
Description: Restored frequency of sipscs, and no effect on amplitude
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla)
|
Whole-cell patch clamp |
2-3 months |
| |
|
Description: Restored frequency of sipscs, and no effect on amplitude
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla) |
|
|
| Neurophysiology |
Miniature post synaptic current amplitude: inhibitory1 |
No adverse effect
Description: No change in amplitude of mipscs compared to parent model or wild type
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla)
|
Whole-cell patch clamp |
2-3 months |
| |
|
Description: No change in amplitude of mipscs compared to parent model or wild type
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla) |
|
|
| Neurophysiology |
Spontaneous post synaptic events: excitatory currents1 |
Refractory
Description: Increased amplitude of sepscs compared to parent model and wild type, ameliorated frequency of sepscs
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla)
|
Whole-cell patch clamp |
2-3 months |
| |
|
Description: Increased amplitude of sepscs compared to parent model and wild type, ameliorated frequency of sepscs
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla) |
|
|
| Neurophysiology |
Miniature post synaptic current amplitude: excitatory1 |
Side effect
Description: Increased amplitude of mepscs compared to parent model
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla)
|
Whole-cell patch clamp |
2-3 months |
| |
|
Description: Increased amplitude of mepscs compared to parent model
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla) |
|
|
| Neurophysiology |
Miniature post synaptic current frequency: inhibitory1 |
Restored
Description: Restored frequency of mipscs, compared to parent model, not changed compared to wild type
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla)
|
Whole-cell patch clamp |
2-3 months |
| |
|
Description: Restored frequency of mipscs, compared to parent model, not changed compared to wild type
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla) |
|
|
| Neurophysiology |
Synaptic plasticity1 |
Restored
Description: Restored long term potentiation in the bla, 50 minutes after tbs stimulation, compared to parent model
Exp Paradigm: Whole-cell patch clamp: current-clamp mode in pyramidal cells of the basolateral amygdala (bla); long term potentiation (ltp) using theta burst stimulation
|
Whole-cell patch clamp |
2-3 months |
| |
|
Description: Restored long term potentiation in the bla, 50 minutes after tbs stimulation, compared to parent model
Exp Paradigm: Whole-cell patch clamp: current-clamp mode in pyramidal cells of the basolateral amygdala (bla); long term potentiation (ltp) using theta burst stimulation |
|
|
| Sensory |
Olfaction1 |
No adverse effect
Description: No change in ability to discriminate odors or to habituate to familiar odors, compared to parent model or wild type
Exp Paradigm: Olfactory discrimination and habituation test
|
Olfactory discrimination test |
Adult |
| |
|
Description: No change in ability to discriminate odors or to habituate to familiar odors, compared to parent model or wild type
Exp Paradigm: Olfactory discrimination and habituation test |
|
|
| Social behavior |
Social memory1 |
Restored
Description: Restored preference for novel mouse at 24-hour test, compared to parent model and no change compared to wild type, and short-term social memory (10-minute test) was not affected
Exp Paradigm: Reciprocal social interaction test: confined stimulus mouse - 24-hour test
|
Reciprocal social interaction test |
Adult |
| |
|
Description: Restored preference for novel mouse at 24-hour test, compared to parent model and no change compared to wild type, and short-term social memory (10-minute test) was not affected
Exp Paradigm: Reciprocal social interaction test: confined stimulus mouse - 24-hour test |
|
|
| Social behavior |
Social approach1 |
No adverse effect
Description: No change in preference for social target, compared to parent model or wild type
Exp Paradigm: Reciprocal social interaction test: confined stimulus mouse
|
Reciprocal social interaction test |
Adult |
| |
|
Description: No change in preference for social target, compared to parent model or wild type
Exp Paradigm: Reciprocal social interaction test: confined stimulus mouse |
|
|
| Emotion |
Depression1 |
Side effect
Description: Decreased despair-associated immobility compared to parent model and wild type
Exp Paradigm: Porsolt forced swim test - 24-hour test
|
Forced swim test |
Adult |
| |
|
Description: Decreased despair-associated immobility compared to parent model and wild type
Exp Paradigm: Porsolt forced swim test - 24-hour test |
|
|
| Emotion |
Anxiety1 |
No adverse effect
Description: No change in time spent in open arms or in number of entries to open arms, compared to parent model
Exp Paradigm: Elevated plus maze over two days
|
Elevated plus maze test |
Adult |
| |
|
Description: No change in time spent in open arms or in number of entries to open arms, compared to parent model
Exp Paradigm: Elevated plus maze over two days |
|
|
| Molecular profile |
Protein expression level evidence1 |
Ameliorated
Description: Restored expression of 3 proteins out of 73 with altered expression in parent model: adam22, cap1, hsp70
Exp Paradigm: Liquid chromatography-mass spectrometry (lc-ms)
|
Liquid chromatography-mass spectrometry (lc-ms) |
Adult |
| |
|
Description: Restored expression of 3 proteins out of 73 with altered expression in parent model: adam22, cap1, hsp70
Exp Paradigm: Liquid chromatography-mass spectrometry (lc-ms) |
|
|
| Molecular profile |
Protein expression level evidence1 |
Restored
Description: Restored number of adam22 immunoreactive cells in the bla
Exp Paradigm: Immunohistochemistry: adam22 antibody
|
Immunohistochemistry |
Adult |
| |
|
Description: Restored number of adam22 immunoreactive cells in the bla
Exp Paradigm: Immunohistochemistry: adam22 antibody |
|
|
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Immune response, Learning & memory, Maternal behavior, Motor phenotype, Neuroanatomy / ultrastructure / cytoarchitecture, Physiological parameters, Repetitive behavior, Seizure, |
