| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
Hyperactivity1 |
Increased
Description: Increased total distance traveled and increased number of ambulatory episodes, compared to nl3-cko littermate controls
Exp Paradigm: Open field test, illuminated 60-minute session
|
Open field test |
6 weeks |
| |
|
Description: Increased total distance traveled and increased number of ambulatory episodes, compared to nl3-cko littermate controls
Exp Paradigm: Open field test, illuminated 60-minute session |
|
|
| Neurophysiology |
Miniature post synaptic current amplitude: excitatory2 |
Decreased
Description: Mutants show decreased amplitude of climbing-fiber excitatory postsynaptic currents compared to controls.
Exp Paradigm: Climbing fibers were identified by their characteristic all-or-none response.
|
Whole-cell current clamp |
1.2 months |
| |
|
Description: Mutants show decreased amplitude of climbing-fiber excitatory postsynaptic currents compared to controls.
Exp Paradigm: Climbing fibers were identified by their characteristic all-or-none response. |
|
|
| Molecular profile |
Targeted expression2 |
Decreased
Description: Mutants show decreased expression of nlgn3 compared to controls.
Exp Paradigm: Calbindin and actin are used as loading controls.
|
Western blot |
Unreported |
| |
|
Description: Mutants show decreased expression of nlgn3 compared to controls.
Exp Paradigm: Calbindin and actin are used as loading controls. |
|
|
| Molecular profile |
Protein expression level evidence2 |
No change |
Western blot |
Unreported |
| Motor phenotype |
Motor coordination and balance1 |
No change |
Accelerating rotarod test |
6 weeks |
| Neurophysiology |
Decay kinetics of miniature post synaptic currents2 |
No change |
Whole-cell current clamp |
P21-25 |
| Neurophysiology |
Intrinsic membrane properties2 |
No change |
Whole-cell patch clamp |
P21-25 |
| Neurophysiology |
Miniature post synaptic current amplitude: inhibitory2 |
No change |
Whole-cell patch clamp |
P21-25 |
| Neurophysiology |
Miniature post synaptic current frequency: inhibitory2 |
No change |
Whole-cell patch clamp |
P21-25 |
| Neurophysiology |
Presynaptic function: paired-pulse facilitation2 |
No change |
Whole-cell current clamp |
1.2 months |
| Neurophysiology |
Spontaneous post synaptic event amplitude: inhibitory currents2 |
No change |
Whole-cell patch clamp |
P21-25 |
| Neurophysiology |
Spontaneous post synaptic events: inhibitory currents2 |
No change |
Whole-cell patch clamp |
P21-25 |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Emotion, Immune response, Learning & memory, Maternal behavior, Molecular profile, Motor phenotype, Neuroanatomy / ultrastructure / cytoarchitecture, Neurophysiology, Physiological parameters, Repetitive behavior, Seizure, Sensory, Social behavior, |
