| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
Motor coordination and balance1 |
Increased
Description: Normal initial coordination with increased learning rate, compared to male littermate controls
Exp Paradigm: Accelerating rotarod, six trials 4 to 40 rpm in 300 s, six more trials 8 to 80 rpm in 300 s
|
Accelerating rotarod test |
6 weeks |
| |
|
Description: Normal initial coordination with increased learning rate, compared to male littermate controls
Exp Paradigm: Accelerating rotarod, six trials 4 to 40 rpm in 300 s, six more trials 8 to 80 rpm in 300 s |
|
|
| Motor phenotype |
Hyperactivity1 |
Increased
Description: Increased total distance traveled and increased number of ambulatory episodes, compared to male littermate controls, no change in speed
Exp Paradigm: Open field test, illuminated 60-minute session
|
Open field test |
6 weeks |
| |
|
Description: Increased total distance traveled and increased number of ambulatory episodes, compared to male littermate controls, no change in speed
Exp Paradigm: Open field test, illuminated 60-minute session |
|
|
| Repetitive behavior |
Stereotypy1 |
Increased
Description: Increased time spent ambulatory and performing stereotypic movements
Exp Paradigm: Open field test, illuminated 60-minute session
|
Open field test |
6 weeks |
| |
|
Description: Increased time spent ambulatory and performing stereotypic movements
Exp Paradigm: Open field test, illuminated 60-minute session |
|
|
| Repetitive behavior |
Perseveration1 |
Increased
Description: Increased repetitive behavior with training by observed reduction of variability of vertical location of each step, step length and time between steps, compared to male littermate controls
Exp Paradigm: Accelerating rotarod, first 30 s of trial 7 and trial 12 (8 to 80 rpm)
|
Accelerating rotarod test |
6 weeks |
| |
|
Description: Increased repetitive behavior with training by observed reduction of variability of vertical location of each step, step length and time between steps, compared to male littermate controls
Exp Paradigm: Accelerating rotarod, first 30 s of trial 7 and trial 12 (8 to 80 rpm) |
|
|
| Physiological parameters |
Digestive system function: gastrointestinal motility: colonic motility2 |
Abnormal
Description: Nlgn3 mutant mice show decreased percentage of forward reverse contraction pattern, decreased cecal-colonic contraction interval.
|
Measurement of GI motility |
8-14 weeks |
| |
|
Description: Nlgn3 mutant mice show decreased percentage of forward reverse contraction pattern, decreased cecal-colonic contraction interval.
|
|
|
| Physiological parameters |
Digestive system function: gastrointestinal motility: peristaltic reflexes2 |
Increased
Description: Nlgn3 knockin mutant mice show increased velocity of forward/peristaltic contractions, decreased duration and decreased start position; no change in velocity of reverse/anti-peristaltic contraction, and decreased duration.
|
Measurement of GI motility |
8-14 weeks |
| |
|
Description: Nlgn3 knockin mutant mice show increased velocity of forward/peristaltic contractions, decreased duration and decreased start position; no change in velocity of reverse/anti-peristaltic contraction, and decreased duration.
|
|
|
| Physiological parameters |
Digestive system function: gastrointestinal motility: intestinal motility2 |
Abnormal
Description: Nlgn3 mutant mice show increased number of cecal motor complexes per 15 minutes, increased velocity, decreased duration, and no change in quiescence.
|
Measurement of GI motility |
8-14 weeks |
| |
|
Description: Nlgn3 mutant mice show increased number of cecal motor complexes per 15 minutes, increased velocity, decreased duration, and no change in quiescence.
|
|
|
| Developmental profile |
Tissue weight2 |
Decreased
Description: Nlgn3 knockin mutants show decreased cecal weight before clearing, decrease content weight, decreased weight content-body weight ratio.
|
Measurement of tissue weight |
8-14 weeks |
| |
|
Description: Nlgn3 knockin mutants show decreased cecal weight before clearing, decrease content weight, decreased weight content-body weight ratio.
|
|
|
| Immune response |
Development of immune cells and primary lymphoid organs2 |
Increased
Description: Nlgn3 knockin mutant mice show increased total cecal patch count. Cecal patches are resident gut-associated lymphoid tissue.
|
Macroscopic analysis |
8-14 weeks |
| |
|
Description: Nlgn3 knockin mutant mice show increased total cecal patch count. Cecal patches are resident gut-associated lymphoid tissue.
|
|
|
| Developmental profile |
Digestive system morphology2 |
No change |
Histology |
8-14 weeks |
| Emotion |
Anxiety1 |
No change |
Open field test |
6 weeks |
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Neuronal number: enteric neurons2 |
No change |
Immunohistochemistry |
8-14 weeks |
| Physiological parameters |
Digestive system function: intestinal barrier: intestinal permeability2 |
No change |
Intestinal permeability test |
8-14 weeks |
| Physiological parameters |
Digestive system function: mucosal morphology: mucosal growth2 |
No change |
Histology |
8-14 weeks |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Immune response, Learning & memory, Maternal behavior, Molecular profile, Neuroanatomy / ultrastructure / cytoarchitecture, Neurophysiology, Physiological parameters, Seizure, Sensory, Social behavior, |
