| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Circadian sleep/wake cycle |
Non rapid eye movement sleep (nrems)1 |
Abnormal
Description: Mutants show reduced theta and alpha powers during nrem sleep, compared to controls. mutants show no change in the proportion of time spent in nrem sleep, compared to controls, in the light or dark period.
Exp Paradigm: Proportion of time that the mice spent in each vigilance state and their distribution during the light and dark periods were recorded. powers of delta (14 hz), theta (58 hz) and alpha (912 hz) frequency bands were examined in each sleep/wake state over time as these are indicators of cortical arousal and sleep quality.
|
Electroencephalogram/electromyogram (eeg/emg) |
4.5 months |
| |
|
Description: Mutants show reduced theta and alpha powers during nrem sleep, compared to controls. mutants show no change in the proportion of time spent in nrem sleep, compared to controls, in the light or dark period.
Exp Paradigm: Proportion of time that the mice spent in each vigilance state and their distribution during the light and dark periods were recorded. powers of delta (14 hz), theta (58 hz) and alpha (912 hz) frequency bands were examined in each sleep/wake state over time as these are indicators of cortical arousal and sleep quality. |
|
|
| Circadian sleep/wake cycle |
Circadian rhythm1 |
Abnormal
Description: Mutants show reduced eeg power between 2 and 8 hz, compared to controls. mutants show no change in the distribution or proportion of time spent in wakefulness compared to controls, in the light or dark period. mutants show increased eeg sigma and beta powers during wakefulness compared to controls.
Exp Paradigm: Proportion of time that the mice spent in each vigilance state and their distribution during the light and dark periods were recorded. powers of delta (14 hz), theta (58 hz) and alpha (912 hz) frequency bands were examined in each sleep/wake state over time as these are indicators of cortical arousal and sleep quality.
|
Electroencephalogram/electromyogram (eeg/emg) |
4.5 months |
| |
|
Description: Mutants show reduced eeg power between 2 and 8 hz, compared to controls. mutants show no change in the distribution or proportion of time spent in wakefulness compared to controls, in the light or dark period. mutants show increased eeg sigma and beta powers during wakefulness compared to controls.
Exp Paradigm: Proportion of time that the mice spent in each vigilance state and their distribution during the light and dark periods were recorded. powers of delta (14 hz), theta (58 hz) and alpha (912 hz) frequency bands were examined in each sleep/wake state over time as these are indicators of cortical arousal and sleep quality. |
|
|
| Circadian sleep/wake cycle |
Sleep pattern1 |
Abnormal
Description: Mutants show no change in the sleep-wake episodes or episode durations in the light or dark phase, compared to controls. mutants show reduced delta power, compared to controls, which is an indicator of sleep depth.
Exp Paradigm: Proportion of time that the mice spent in each vigilance state and their distribution during the light and dark periods were recorded. powers of delta (14 hz), theta (58 hz) and alpha (912 hz) frequency bands were examined in each sleep/wake state over time as these are indicators of cortical arousal and sleep quality.
|
Electroencephalogram/electromyogram (eeg/emg) |
4.5 months |
| |
|
Description: Mutants show no change in the sleep-wake episodes or episode durations in the light or dark phase, compared to controls. mutants show reduced delta power, compared to controls, which is an indicator of sleep depth.
Exp Paradigm: Proportion of time that the mice spent in each vigilance state and their distribution during the light and dark periods were recorded. powers of delta (14 hz), theta (58 hz) and alpha (912 hz) frequency bands were examined in each sleep/wake state over time as these are indicators of cortical arousal and sleep quality. |
|
|
| Circadian sleep/wake cycle |
Rapid eye movement sleep (rems)1 |
Abnormal
Description: Mutants show reduced alpha power during rem sleep compared to controls. mutants show no change in the proportion of time spent in rem sleep, compared to controls, in the light or dark period.
Exp Paradigm: Proportion of time that the mice spent in each vigilance state and their distribution during the light and dark periods were recorded. powers of delta (14 hz), theta (58 hz) and alpha (912 hz) frequency bands were examined in each sleep/wake state over time as these are indicators of cortical arousal and sleep quality.
|
Electroencephalogram/electromyogram (eeg/emg) |
4.5 months |
| |
|
Description: Mutants show reduced alpha power during rem sleep compared to controls. mutants show no change in the proportion of time spent in rem sleep, compared to controls, in the light or dark period.
Exp Paradigm: Proportion of time that the mice spent in each vigilance state and their distribution during the light and dark periods were recorded. powers of delta (14 hz), theta (58 hz) and alpha (912 hz) frequency bands were examined in each sleep/wake state over time as these are indicators of cortical arousal and sleep quality. |
|
|
| Motor phenotype |
General locomotor activity2 |
Decreased
Description: Decreased rate of rearing (vertical) activity in nl3 male mice compared to wild types
Exp Paradigm: Open field test
|
Open field test |
Adult |
| |
|
Description: Decreased rate of rearing (vertical) activity in nl3 male mice compared to wild types
Exp Paradigm: Open field test |
|
|
| Motor phenotype |
Righting response2 |
Decreased
Description: Delayed righting response in males and females
Exp Paradigm: Righting response test
|
Righting reflex test |
P2-p6 |
| |
|
Description: Delayed righting response in males and females
Exp Paradigm: Righting response test |
|
|
| Motor phenotype |
Gait2 |
Abnormal
Description: Increased forepaw width, but no change in hindpaw width or stride length in nl3 females compared to wild types
Exp Paradigm: Footprint pattern analysis
|
Footprint analysis |
Adult |
| |
|
Description: Increased forepaw width, but no change in hindpaw width or stride length in nl3 females compared to wild types
Exp Paradigm: Footprint pattern analysis |
|
|
| Motor phenotype |
Motor coordination and balance2 |
Increased
Description: Increased latency to fall in male nl3 mice
Exp Paradigm: Accelerating rota rod test
|
Accelerating rotarod test |
Adult |
| |
|
Description: Increased latency to fall in male nl3 mice
Exp Paradigm: Accelerating rota rod test |
|
|
| Motor phenotype |
Grip strength2 |
Decreased
Description: Shorter latency to fall in the wire hang test for nl3 males compared to wild types
Exp Paradigm: Wire hang test
|
Wire hang test |
Adult |
| |
|
Description: Shorter latency to fall in the wire hang test for nl3 males compared to wild types
Exp Paradigm: Wire hang test |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Astrocyte morphology4 |
Decreased
Description: Decrease in astrocyte branch length, the number of branch points, and cell radius and area in the dentate gyrus; no change in astrocyte morphology in the ca1
|
Immunofluorescence staining |
2-5 months |
| |
|
Description: Decrease in astrocyte branch length, the number of branch points, and cell radius and area in the dentate gyrus; no change in astrocyte morphology in the ca1
|
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Microglial number4 |
Increased
Description: Increased microglial density in the dentate gyrus but not hippocampus ca1 region
|
Immunofluorescence staining |
2-5 months |
| |
|
Description: Increased microglial density in the dentate gyrus but not hippocampus ca1 region
|
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Microglial morphology4 |
Abnormal
Description: No change in microglial morphology in the dentate gyrus; microglia somata were elongated in the ca1; soma eccentricity was increased in the ca1 region
|
Immunofluorescence staining |
2-5 months |
| |
|
Description: No change in microglial morphology in the dentate gyrus; microglia somata were elongated in the ca1; soma eccentricity was increased in the ca1 region
|
|
|
| Neurophysiology |
Synaptic transmission: excitatory3 |
Increased
Description: Increased fepsp, compared to wild type
Exp Paradigm: Field potential recordings: slope of epsp by range of stimulus (input/ output relationship)
|
Field potential recordings |
Unreported |
| |
|
Description: Increased fepsp, compared to wild type
Exp Paradigm: Field potential recordings: slope of epsp by range of stimulus (input/ output relationship) |
|
|
| Sensory |
Startle response: acoustic stimulus2 |
Decreased
Description: Decreased startle response to 100-db, 110-db and 120-db acoustic stimuli
Exp Paradigm: Acoustic startle
|
Prepulse inhibition |
Adult |
| |
|
Description: Decreased startle response to 100-db, 110-db and 120-db acoustic stimuli
Exp Paradigm: Acoustic startle |
|
|
| Social behavior |
Social scent marking or recognition2 |
Increased
Description: In females, shorter latency to reach nest area, nl3 females compared to wild types
Exp Paradigm: Homing test, females
|
Homing test |
P9 |
| |
|
Description: In females, shorter latency to reach nest area, nl3 females compared to wild types
Exp Paradigm: Homing test, females |
|
|
| Communications |
Ultrasonic vocalization2 |
Decreased
Description: Decreased ultrasonic vocalization calls in male pups, but no change in duration, peak frequency or amplitude of calls, compared to wild types
Exp Paradigm: Maternal separation
|
Monitoring ultrasonic vocalizations |
P8 |
| |
|
Description: Decreased ultrasonic vocalization calls in male pups, but no change in duration, peak frequency or amplitude of calls, compared to wild types
Exp Paradigm: Maternal separation |
|
|
| Developmental profile |
Size/growth2 |
Increased
Description: Increased body weight in male nl3 pups, compared to wild type
Exp Paradigm: General observations
|
General observations |
P6-p11 |
| |
|
Description: Increased body weight in male nl3 pups, compared to wild type
Exp Paradigm: General observations |
|
|
| Developmental profile |
Size/growth2 |
Decreased
Description: Decreased body weight in nl3 females, compared to wild types
Exp Paradigm: General observations
|
General observations |
Adult |
| |
|
Description: Decreased body weight in nl3 females, compared to wild types
Exp Paradigm: General observations |
|
|
| Developmental profile |
Size/growth2 |
Decreased
Description: Decreased body weight in female nl3 pups, compared to wild type
Exp Paradigm: General observations
|
General observations |
P6-p12 |
| |
|
Description: Decreased body weight in female nl3 pups, compared to wild type
Exp Paradigm: General observations |
|
|
| Molecular profile |
Protein expression: In situ protein expression4 |
Abnormal
Description: Increased striatal postsynaptic density 95 (psd-95) protein levels; decreased cortical expression of synaptosomal-associated protein 25 (snap-25); no change in gfap expression in cortex, striatum or cerebellum
|
Western blot |
3 months |
| |
|
Description: Increased striatal postsynaptic density 95 (psd-95) protein levels; decreased cortical expression of synaptosomal-associated protein 25 (snap-25); no change in gfap expression in cortex, striatum or cerebellum
|
|
|
| Molecular profile |
Targeted expression4 |
Decreased
Description: Neuroligin 3 protein expression was dramatically decreased in cortical, striatal and cerebellar tissue samples
|
Western blot |
3 months |
| |
|
Description: Neuroligin 3 protein expression was dramatically decreased in cortical, striatal and cerebellar tissue samples
|
|
|
| Communications |
Ultrasonic vocalization2 |
No change |
Monitoring ultrasonic vocalizations |
P2-p11 |
| Developmental profile |
Size/growth2 |
No change |
General observations |
Adult |
| Emotion |
Anxiety2 |
No change |
Elevated plus maze test |
Adult |
| Learning & memory |
Cognitive flexibility2 |
No change |
Morris water maze test |
Adult |
| Learning & memory |
Cued or contextual fear conditioning2 |
No change |
Fear conditioning test |
Adult |
| Learning & memory |
Spatial learning2 |
No change |
Morris water maze test |
Adult |
| Learning & memory |
Spatial reference memory2 |
No change |
Morris water maze test |
Adult |
| Motor phenotype |
Gait2 |
No change |
Footprint analysis |
Adult |
| Motor phenotype |
General locomotor activity2 |
No change |
Open field test |
Adult |
| Motor phenotype |
General locomotor activity: ambulatory activity2 |
No change |
Open field test |
Adult |
| Motor phenotype |
Motor coordination and balance2 |
No change |
Accelerating rotarod test |
Adult |
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Astrocyte number4 |
No change |
Immunofluorescence staining |
2-5 months |
| Sensory |
Hearing2 |
No change |
Prepulse inhibition |
Adult |
| Sensory |
Sensorimotor gating2 |
No change |
Prepulse inhibition |
Adult |
| Social behavior |
Social approach2 |
No change |
Three-chamber social approach test |
Adult |
| Social behavior |
Social interaction2 |
No change |
Reciprocal social interaction test |
P19-p23 |
| Social behavior |
Social scent marking or recognition2 |
No change |
Homing test |
P9 |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Emotion, Immune response, Learning & memory, Maternal behavior, Molecular profile, Motor phenotype, Neuroanatomy / ultrastructure / cytoarchitecture, Neurophysiology, Physiological parameters, Repetitive behavior, Seizure, Sensory, Social behavior, |
