| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
Motor coordination and balance1 |
Increased
Description: increase in latency to fall off
|
Accelerating rotarod test |
Adult |
| |
|
Description: increase in latency to fall off
|
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Synapse density1 |
Decreased
Description: impairment of the canonical and non-canonical synaptogenic pathways in these mutant mice by coculture assays using NRXN1β and PTPδA3Bâ?? beads
|
Immunostaining |
Adult |
| |
|
Description: impairment of the canonical and non-canonical synaptogenic pathways in these mutant mice by coculture assays using NRXN1β and PTPδA3Bâ?? beads
|
|
|
| Neurophysiology |
Miniature post synaptic current frequency: inhibitory1 |
Increased
Description: frequencies of both miniature inhibitory postsynaptic currents (mIPSC) were increased
|
Whole-cell patch clamp |
Adult |
| |
|
Description: frequencies of both miniature inhibitory postsynaptic currents (mIPSC) were increased
|
|
|
| Neurophysiology |
Miniature post synaptic current frequency: excitatory1 |
Increased
Description: frequencies of both miniature excitatory postsynaptic currents (mEPSC) were increased
|
Whole-cell patch clamp |
Adult |
| |
|
Description: frequencies of both miniature excitatory postsynaptic currents (mEPSC) were increased
|
|
|
| Social behavior |
Social interaction1 |
Increased
Description: mutant mice spent more time in proximity with the other mice than their wild-type littermates; increase in number of head to head contacts; shorter duration in each stranger-cage-sniffing behavior than their wild-type littermates
|
Reciprocal social interaction test |
Adult |
| |
|
Description: mutant mice spent more time in proximity with the other mice than their wild-type littermates; increase in number of head to head contacts; shorter duration in each stranger-cage-sniffing behavior than their wild-type littermates
|
|
|
| Molecular profile |
Protein binding1 |
Abnormal
Description: in cortical neurons from Nlgn3mf male mice, PTPδA3Bâ?? beads failed to induce NLGN3, Shank2, and gephyrin accumulation, while NRXN1β-induced NLGN3 and gephyrin accumulation was markedly enhanced
|
Immunostaining |
Adult |
| |
|
Description: in cortical neurons from Nlgn3mf male mice, PTPδA3Bâ?? beads failed to induce NLGN3, Shank2, and gephyrin accumulation, while NRXN1β-induced NLGN3 and gephyrin accumulation was markedly enhanced
|
|
|
| Molecular profile |
Protein binding1 |
Decreased
Description: anti-NLGN3 antibody failed to coimmunoprecipitate PTPδ in the Nlgn3mf mice brain, indicating the non-canonical NLGN3â??PTPδ transsynaptic pathway is impaired
|
Co-immunoprecipitation |
Adult |
| |
|
Description: anti-NLGN3 antibody failed to coimmunoprecipitate PTPδ in the Nlgn3mf mice brain, indicating the non-canonical NLGN3â??PTPδ transsynaptic pathway is impaired
|
|
|
| Molecular profile |
Protein expression: In situ protein expression1 |
Decreased
Description: the expression of inhibitory synaptic proteins vesicular GABA transporter (VGAT) and gephyrin, was selectively upregulated, while those of the excitatory synaptic proteins vesicular glutamate transporter 1 (VGluT1) and PSD95 remained unaltered; immunostaining signal ratios for VGAT compared to VGluT1 in the cerebral cortex were higher
|
Whole-cell patch clamp |
Adult |
| |
|
Description: the expression of inhibitory synaptic proteins vesicular GABA transporter (VGAT) and gephyrin, was selectively upregulated, while those of the excitatory synaptic proteins vesicular glutamate transporter 1 (VGluT1) and PSD95 remained unaltered; immunostaining signal ratios for VGAT compared to VGluT1 in the cerebral cortex were higher
|
|
|
| Molecular profile |
Protein degradation: proteasome1 |
No change |
Immunostaining |
Adult |
| Social behavior |
Social approach1 |
No change |
Three-chamber social approach test |
Adult |
|
| Not Reported: |
|
