| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Synapse density1 |
Decreased
Description: impairment of the canonical and non-canonical synaptogenic pathways in these mutant mice by coculture assays using NRXN1β and PTPδA3Bâ?? beads
|
Immunostaining |
Adult |
| |
|
Description: impairment of the canonical and non-canonical synaptogenic pathways in these mutant mice by coculture assays using NRXN1β and PTPδA3Bâ?? beads
|
|
|
| Social behavior |
Social interaction1 |
Increased
Description: mutant mice spent more time in proximity with the other mice than their wild-type littermates; no change in number of counts of defensive upright posture measured by elevated head-head contacts throughout the test period
|
Reciprocal social interaction test |
Adult |
| |
|
Description: mutant mice spent more time in proximity with the other mice than their wild-type littermates; no change in number of counts of defensive upright posture measured by elevated head-head contacts throughout the test period
|
|
|
| Social behavior |
Social approach1 |
Increased
Description: mutants and their wild-type littermates spent more time in the chamber with the stranger mouse than in the chamber with the non-social empty cage, suggesting that disruption of the canonical NLGN3â??NRXN interaction enhances sociability development
|
Three-chamber social approach test |
Adult |
| |
|
Description: mutants and their wild-type littermates spent more time in the chamber with the stranger mouse than in the chamber with the non-social empty cage, suggesting that disruption of the canonical NLGN3â??NRXN interaction enhances sociability development
|
|
|
| Molecular profile |
Protein binding1 |
Abnormal
Description: in cortical neurons from Nlgn3hse mutant male mice, NRXN1β-beads failed to induce accumulation of NLGN3 while Shank2 and gephyrin signals on the NRXN1β-beads were attenuated but not completely abolished ; PTPδA3Bâ??-mediated accumulation of NLGN3 and gephyrin, but not Shank2, was upregulated
|
Immunostaining |
Adult |
| |
|
Description: in cortical neurons from Nlgn3hse mutant male mice, NRXN1β-beads failed to induce accumulation of NLGN3 while Shank2 and gephyrin signals on the NRXN1β-beads were attenuated but not completely abolished ; PTPδA3Bâ??-mediated accumulation of NLGN3 and gephyrin, but not Shank2, was upregulated
|
|
|
| Molecular profile |
Protein binding1 |
Increased
Description: anti-NLGN3 antibody coimmunoprecipitated more PTPδ in the brains of the Nlgn3hse mutants than in those of wild-type littermates, indicating the canonical NLGN3â??NRXN transsynaptic pathway is impaired
|
Co-immunoprecipitation |
Adult |
| |
|
Description: anti-NLGN3 antibody coimmunoprecipitated more PTPδ in the brains of the Nlgn3hse mutants than in those of wild-type littermates, indicating the canonical NLGN3â??NRXN transsynaptic pathway is impaired
|
|
|
| Molecular profile |
Protein degradation: proteasome1 |
No change |
Immunostaining |
Adult |
| Molecular profile |
Protein expression: In situ protein expression1 |
No change |
Western blot |
Adult |
| Motor phenotype |
Motor coordination and balance1 |
No change |
Accelerating rotarod test |
Adult |
| Neurophysiology |
Synaptic transmission: inhibitory1 |
No change |
Whole-cell patch clamp |
Adult |
|
| Not Reported: |
|
