| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Neurophysiology |
Synaptic plasticity: ventral tegmental area ltp1 |
Decreased
Description: Vta specific nlgn3 knockdown mutant mice show lack of synaptic plasticity induced by exposure to nonfamiliar conspecifics compared with controls. glua2-lacking ampars in vta, abnormally elevated in dopaminergic vta specific nlgn3 knockdown mutants show no further increase 24 hours after exposure to nonfamiliar social stimulus, indicating glua2 lacking ampars in vta da neurons is associated with impaired response to novel social stimulus.
Exp Paradigm: NA
|
Whole-cell patch clamp |
4-8 weeks |
| |
|
Description: Vta specific nlgn3 knockdown mutant mice show lack of synaptic plasticity induced by exposure to nonfamiliar conspecifics compared with controls. glua2-lacking ampars in vta, abnormally elevated in dopaminergic vta specific nlgn3 knockdown mutants show no further increase 24 hours after exposure to nonfamiliar social stimulus, indicating glua2 lacking ampars in vta da neurons is associated with impaired response to novel social stimulus.
Exp Paradigm: NA |
|
|
| Neurophysiology |
Neuroreceptor activity1 |
Abnormal
Description: Vta specific nlgn3 knockdown mutant mice show increase in rectification index of ampar mediated currents compared with controls, indicating aberrant increase of glua2-lacking ampars in nlgn3 deficient vta da neurons.
Exp Paradigm: NA
|
Whole-cell patch clamp |
4-8 weeks |
| |
|
Description: Vta specific nlgn3 knockdown mutant mice show increase in rectification index of ampar mediated currents compared with controls, indicating aberrant increase of glua2-lacking ampars in nlgn3 deficient vta da neurons.
Exp Paradigm: NA |
|
|
| Social behavior |
Social habituation1 |
Decreased
Description: Mutants show overall lower interaction times and no significant habituation compared with controls.
Exp Paradigm: NA
|
Habituation-dishabituation test |
2 months |
| |
|
Description: Mutants show overall lower interaction times and no significant habituation compared with controls.
Exp Paradigm: NA |
|
|
| Social behavior |
Social dishabituation1 |
Decreased
Description: Mutants show no increase in interaction in response to the nonfamiliar conspecific mice as seen in controls.
Exp Paradigm: NA
|
Habituation-dishabituation test |
2 months |
| |
|
Description: Mutants show no increase in interaction in response to the nonfamiliar conspecific mice as seen in controls.
Exp Paradigm: NA |
|
|
| Learning & memory |
Reward reinforced choice behavior1 |
Decreased
Description: Mutants did not develop a preference for the social compartments when conditioned in a conditioned place preference paradigm with a familiar mouse, compared with controls.
Exp Paradigm: NA
|
Conditioned place preference test |
1.2-1.5 months |
| |
|
Description: Mutants did not develop a preference for the social compartments when conditioned in a conditioned place preference paradigm with a familiar mouse, compared with controls.
Exp Paradigm: NA |
|
|
| Molecular profile |
Targeted expression1 |
Decreased
Description: Mutants show decrease in nlgn3 protein expression in cortical neurons compared with controls.
Exp Paradigm: NA
|
Western blot |
~11 weeks |
| |
|
Description: Mutants show decrease in nlgn3 protein expression in cortical neurons compared with controls.
Exp Paradigm: NA |
|
|
| Molecular profile |
Targeted expression1 |
Decreased
Description: Mutants show gfp immunofluorescence in infected neurons in the vta compared with controls. tyrosine hydroxylase positive da neurons infected with aav2-dio-mirnlgn3 are detected in the vta, substantia nigra pars compacta (snc), dorsal raphe nucleus and retrorubral area (rr).
Exp Paradigm: NA
|
Immunohistochemistry |
~11 weeks |
| |
|
Description: Mutants show gfp immunofluorescence in infected neurons in the vta compared with controls. tyrosine hydroxylase positive da neurons infected with aav2-dio-mirnlgn3 are detected in the vta, substantia nigra pars compacta (snc), dorsal raphe nucleus and retrorubral area (rr).
Exp Paradigm: NA |
|
|
| Learning & memory |
Object recognition memory1 |
No change |
Novel object recognition test |
1.7 months |
| Learning & memory |
Olfactory learning and memory1 |
No change |
Olfactory discrimination test |
Not specified |
| Molecular profile |
Protein expression level evidence1 |
No change |
Western blot |
~11 weeks |
| Motor phenotype |
General locomotor activity1 |
No change |
Open field test |
1.7 months |
| Repetitive behavior |
Repetitive digging1 |
No change |
Marble-burying test |
2.7 months |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Emotion, Immune response, Maternal behavior, Neuroanatomy / ultrastructure / cytoarchitecture, Physiological parameters, Seizure, Sensory, |
