| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Neurophysiology |
Spontaneous post synaptic event amplitude: excitatory currents1 |
No adverse effect
Description: Mutants show no change in reduction of epsc amplitude compared to wildtype controls, upon perfusion of dorsal striatum with the cb1 receptor agonist hu- 210. cb1 receptor activation did not alter the mean amplitude of sepscs recorded from wt spns.
Exp Paradigm: Corticostrial slices were used for recordings.
|
Whole-cell patch clamp |
2 month |
| |
|
Description: Mutants show no change in reduction of epsc amplitude compared to wildtype controls, upon perfusion of dorsal striatum with the cb1 receptor agonist hu- 210. cb1 receptor activation did not alter the mean amplitude of sepscs recorded from wt spns.
Exp Paradigm: Corticostrial slices were used for recordings. |
|
|
| Neurophysiology |
Presynaptic function: paired-pulse facilitation1 |
No adverse effect
Description: Mutants treated with hu-210 show no change in ppr compared to untreated mutants indicating presynaptic inhibitory effect of cb1 receptor activation on corticostriatal glutamate release is maintained in mutants.
Exp Paradigm: Corticostrial slices were used for recordings.
|
Whole-cell patch clamp |
2 month |
| |
|
Description: Mutants treated with hu-210 show no change in ppr compared to untreated mutants indicating presynaptic inhibitory effect of cb1 receptor activation on corticostriatal glutamate release is maintained in mutants.
Exp Paradigm: Corticostrial slices were used for recordings. |
|
|
| Neurophysiology |
Synaptic plasticity: striatal ltd1 |
Ameliorated
Description: Cb1 receptor activation resulted in a similar inhibitory effect on epsp amplitude in both mutants and controls. hfs delivery during perfusion with hu-210 induced a ltd of corticostriatal transmission in wt slices. hfs delivery to r451c-nl3 slices during exogenous activation of cb1 receptors resulted in a partial rescue of ltd expression.
Exp Paradigm: Corticostriatal slices were perfused for 30 minutes with 1 m hu-210, before hfs delivery.
|
Whole-cell patch clamp |
2 month |
| |
|
Description: Cb1 receptor activation resulted in a similar inhibitory effect on epsp amplitude in both mutants and controls. hfs delivery during perfusion with hu-210 induced a ltd of corticostriatal transmission in wt slices. hfs delivery to r451c-nl3 slices during exogenous activation of cb1 receptors resulted in a partial rescue of ltd expression.
Exp Paradigm: Corticostriatal slices were perfused for 30 minutes with 1 m hu-210, before hfs delivery. |
|
|
| Neurophysiology |
Spontaneous post synaptic event frequency: excitatory currents1 |
No adverse effect
Description: Mutants show no change in epsc frequency compared to wildtype controls, upon perfusion of dorsal striatum with the cb1 receptor agonist hu- 210.
Exp Paradigm: Corticostrial slices were used for recordings.
|
Whole-cell patch clamp |
2 month |
| |
|
Description: Mutants show no change in epsc frequency compared to wildtype controls, upon perfusion of dorsal striatum with the cb1 receptor agonist hu- 210.
Exp Paradigm: Corticostrial slices were used for recordings. |
|
|
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Emotion, Immune response, Learning & memory, Maternal behavior, Molecular profile, Motor phenotype, Neuroanatomy / ultrastructure / cytoarchitecture, Physiological parameters, Repetitive behavior, Seizure, Sensory, Social behavior, |
