| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
General locomotor activity: ambulatory activity1 |
Increased
Description: Mutants travel greater distance than controls.
Exp Paradigm: NA
|
Open field test |
3-4 weeks, 2-4 months |
| |
|
Description: Mutants travel greater distance than controls.
Exp Paradigm: NA |
|
|
| Repetitive behavior |
Repetitive digging1 |
Increased
Description: Mutants from mixed genotype housing buried a larger number of marbles than mutants or wildtype in single genotype housing but buried less marbles than wildtype in mixed genotype housing.
Exp Paradigm: NA
|
Marble-burying test |
2-4 month |
| |
|
Description: Mutants from mixed genotype housing buried a larger number of marbles than mutants or wildtype in single genotype housing but buried less marbles than wildtype in mixed genotype housing.
Exp Paradigm: NA |
|
|
| Social behavior |
Social dominance1 |
Decreased
Description: Mutant males from mixed group housing are pushed out of the tube with greater frequency compared to wildtype males from mixed group housing. mutant males from mgh show an absence of correlation between social rank as determined by the tube test and the number of ultrasonic vocalization whereas mutant males from sgh show a linear correlation between social rank and usv. a structured social hierarchy did not develop in mutant mice from mgh.
Exp Paradigm: NA
|
Tube test of social dominance |
2-4 month |
| |
|
Description: Mutant males from mixed group housing are pushed out of the tube with greater frequency compared to wildtype males from mixed group housing. mutant males from mgh show an absence of correlation between social rank as determined by the tube test and the number of ultrasonic vocalization whereas mutant males from sgh show a linear correlation between social rank and usv. a structured social hierarchy did not develop in mutant mice from mgh.
Exp Paradigm: NA |
|
|
| Social behavior |
Social scent marking or recognition1 |
Increased
Description: Mutant males from mgh showed increased interest toward social odors than non-social odors compared to control male mice in mgh.
Exp Paradigm: NA
|
Olfactory discrimination test |
2-4 month |
| |
|
Description: Mutant males from mgh showed increased interest toward social odors than non-social odors compared to control male mice in mgh.
Exp Paradigm: NA |
|
|
| Social behavior |
Social interaction: with juveniles1 |
Decreased
Description: Juvenile mutant mice from mixed genotype housing spent less time interacting with an unfamiliar adult female in juvenile mice, compared to controls housed in single genotypes.
Exp Paradigm: NA
|
Reciprocal social interaction test |
3-4 weeks |
| |
|
Description: Juvenile mutant mice from mixed genotype housing spent less time interacting with an unfamiliar adult female in juvenile mice, compared to controls housed in single genotypes.
Exp Paradigm: NA |
|
|
| Communications |
Ultrasonic vocalization: interaction induced: opposite sex stimulus1 |
Decreased
Description: Mutant males mice from mixed group housing (mgh) show decreased ultrasonic vocalization during courtship than wildtype males from mixed group housing. mutant males from mgh and sgh spent similar times emitting usvs in the presence of a female in estrus.
Exp Paradigm: NA
|
Monitoring ultrasonic vocalizations |
2-4 month |
| |
|
Description: Mutant males mice from mixed group housing (mgh) show decreased ultrasonic vocalization during courtship than wildtype males from mixed group housing. mutant males from mgh and sgh spent similar times emitting usvs in the presence of a female in estrus.
Exp Paradigm: NA |
|
|
| Molecular profile |
Targeted expression1 |
Decreased
Description: Mutants show decreased expression of nlgn3 protein in the cerebellum, cortex, striatum, hippocampus, brainstem and thalamus compared to controls.
Exp Paradigm: NA
|
Western blot |
2-4 month |
| |
|
Description: Mutants show decreased expression of nlgn3 protein in the cerebellum, cortex, striatum, hippocampus, brainstem and thalamus compared to controls.
Exp Paradigm: NA |
|
|
| Circadian sleep/wake cycle |
Adaptation to dark phase1 |
No change |
Home cage behavior |
2-4 month |
| Emotion |
Anxiety1 |
No change |
Elevated plus maze test |
3-4 weeks, 2-4 months |
| Emotion |
Anxiety1 |
No change |
Open field test |
3-4 weeks, 2-4 months |
| Emotion |
Thigmotaxis1 |
No change |
Open field test |
2-4 month |
| Molecular profile |
Gene expression1 |
No change |
Quantitative pcr (qrt-pcr) |
2-4 month |
| Motor phenotype |
Spontaneous movement1 |
No change |
Spontaneous movement analysis |
2-4 month |
| Physiological parameters |
Hormone levels1 |
No change |
Elisa |
2-4 month |
|
| Not Reported: |
Developmental profile, Immune response, Learning & memory, Maternal behavior, Neuroanatomy / ultrastructure / cytoarchitecture, Neurophysiology, Physiological parameters, Seizure, Sensory, |
