| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
Motor coordination and balance1 |
Restored
Description: Reexpression of nlgn3 specifically in purkinje cells restored motor coordination compared to controls measured by the decreased step time but the number of missteps remained high.
Exp Paradigm: Number of runs, missteps and step time was measured.
|
Erasmus ladder test |
2-3 months |
| |
|
Description: Reexpression of nlgn3 specifically in purkinje cells restored motor coordination compared to controls measured by the decreased step time but the number of missteps remained high.
Exp Paradigm: Number of runs, missteps and step time was measured. |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Neuroreceptor levels: glutamate receptors: nmda receptors1 |
No adverse effect
Description: Reexpression of nlgn3 specifically in purkinje cells did not alter the expression of glud2 or glua2/a3 compared to mutants.
Exp Paradigm: NA
|
Western blot |
2-3 months |
| |
|
Description: Reexpression of nlgn3 specifically in purkinje cells did not alter the expression of glud2 or glua2/a3 compared to mutants.
Exp Paradigm: NA |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Anatomical projections and connectivity1 |
Restored
Description: Reexpression of nlgn3 specifically in purkinje cells suppressed ectopic synapse formation in the distal molecular layer, compared to controls.
Exp Paradigm: Vglut2 positive synapses were counted on purkinje cells and climbing fibers.
|
Histology |
2-3 months |
| |
|
Description: Reexpression of nlgn3 specifically in purkinje cells suppressed ectopic synapse formation in the distal molecular layer, compared to controls.
Exp Paradigm: Vglut2 positive synapses were counted on purkinje cells and climbing fibers. |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Neuroreceptor levels: glutamate receptors: nmda receptors1 |
Restored
Description: Reexpression of nlgn3 specifically in purkinje cells reduced the mglur1a protein level and its synaptic abundance to the wild-type levels.
Exp Paradigm: Western blot
|
Western blot |
2-3 months |
| |
|
Description: Reexpression of nlgn3 specifically in purkinje cells reduced the mglur1a protein level and its synaptic abundance to the wild-type levels.
Exp Paradigm: Western blot |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Neuroreceptor levels: glutamate receptors: nmda receptors1 |
Restored
Description: Reexpression of nlgn3 specifically in purkinje cells reduced the mglur1a protein level and its synaptic abundance to the wild-type levels.
Exp Paradigm: Quantitative pcr (qrt-pcr)
|
Quantitative pcr (qrt-pcr) |
2-3 months |
| |
|
Description: Reexpression of nlgn3 specifically in purkinje cells reduced the mglur1a protein level and its synaptic abundance to the wild-type levels.
Exp Paradigm: Quantitative pcr (qrt-pcr) |
|
|
| Molecular profile |
Protein expression level evidence1 |
No adverse effect
Description: Reexpression of nlgn3 specifically in purkinje cells did not alter the expression of homer1 compared to mutants.
Exp Paradigm: Western blot
|
Western blot |
2-3 months |
| |
|
Description: Reexpression of nlgn3 specifically in purkinje cells did not alter the expression of homer1 compared to mutants.
Exp Paradigm: Western blot |
|
|
| Molecular profile |
Targeted expression1 |
Restored
Description: Reexpression of nlgn3 specifically in purkinje cells increased nlgn3 protein expression in purkinjee cell dendrites in the cerebellum but not in the inner granular layer, compared to mutants. the reexpression of nlgn3 in pcs exceeds the endogenous nlgn3 expression level and results in a gain-of-function phenotype.
Exp Paradigm: Calbindin is used to co-stain for dendrite identification.- histology
|
Histology |
2-3 months |
| |
|
Description: Reexpression of nlgn3 specifically in purkinje cells increased nlgn3 protein expression in purkinjee cell dendrites in the cerebellum but not in the inner granular layer, compared to mutants. the reexpression of nlgn3 in pcs exceeds the endogenous nlgn3 expression level and results in a gain-of-function phenotype.
Exp Paradigm: Calbindin is used to co-stain for dendrite identification.- histology |
|
|
| Molecular profile |
Protein expression level evidence1 |
No adverse effect
Description: Reexpression of nlgn3 specifically in purkinje cells did not alter the expression of homer1 compared to mutants.
Exp Paradigm: Histology
|
Histology |
2-3 months |
| |
|
Description: Reexpression of nlgn3 specifically in purkinje cells did not alter the expression of homer1 compared to mutants.
Exp Paradigm: Histology |
|
|
| Molecular profile |
Protein phosphorylation1 |
Restored
Description: Reexpression of nlgn3 specifically in purkinje cells returned dhpg-induced phospho-glua2 signals to wildtype control levels.
Exp Paradigm: Dhpg, a potent agonist of group i metabotropic glutamate receptors mglur1 and mglur5, is used in a functional assay.
|
Western blot |
3-4 weeks |
| |
|
Description: Reexpression of nlgn3 specifically in purkinje cells returned dhpg-induced phospho-glua2 signals to wildtype control levels.
Exp Paradigm: Dhpg, a potent agonist of group i metabotropic glutamate receptors mglur1 and mglur5, is used in a functional assay. |
|
|
| Molecular profile |
Protein localization: synapse1 |
Restored
Description: Reexpression of nlgn3 specifically in purkinje cells localizes the nlgn3 protein to vglut1 positive purkinje fiber boutons where nlgn3 protein colocalizes with mglur1alpha in calbindin positive spines and in glomeruli, nlgn3 localizes at psd95 positive and gaba-a receptor a2 positive synapses and colocalizes with gfp+ terminals in glyt2-gfp transgenic mice, compared to mutants.
Exp Paradigm: Calbindin is used to co-stain for dendrite identification.
|
Histology |
2-3 months |
| |
|
Description: Reexpression of nlgn3 specifically in purkinje cells localizes the nlgn3 protein to vglut1 positive purkinje fiber boutons where nlgn3 protein colocalizes with mglur1alpha in calbindin positive spines and in glomeruli, nlgn3 localizes at psd95 positive and gaba-a receptor a2 positive synapses and colocalizes with gfp+ terminals in glyt2-gfp transgenic mice, compared to mutants.
Exp Paradigm: Calbindin is used to co-stain for dendrite identification. |
|
|
| Molecular profile |
Targeted expression1 |
Restored
Description: Reexpression of nlgn3 specifically in purkinje cells increased nlgn3 protein expression in purkinjee cell dendrites in the cerebellum but not in the inner granular layer, compared to mutants. the reexpression of nlgn3 in pcs exceeds the endogenous nlgn3 expression level and results in a gain-of-function phenotype.
Exp Paradigm: Calbindin is used to co-stain for dendrite identification.-western blot
|
Western blot |
2-3 months |
| |
|
Description: Reexpression of nlgn3 specifically in purkinje cells increased nlgn3 protein expression in purkinjee cell dendrites in the cerebellum but not in the inner granular layer, compared to mutants. the reexpression of nlgn3 in pcs exceeds the endogenous nlgn3 expression level and results in a gain-of-function phenotype.
Exp Paradigm: Calbindin is used to co-stain for dendrite identification.-western blot |
|
|
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Emotion, Immune response, Learning & memory, Maternal behavior, Neurophysiology, Physiological parameters, Repetitive behavior, Seizure, Sensory, Social behavior, |
