| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Neurophysiology |
Synaptic plasticity1 |
Decreased
Description: Impaired long term potentiation in the bla, 50 minutes after tbs stimulation
Exp Paradigm: Whole-cell patch clamp: current-clamp mode in pyramidal cells of the basolateral amygdala (bla); long term potentiation (ltp) using theta burst stimulation
|
Whole-cell patch clamp |
2-3 months |
| |
|
Description: Impaired long term potentiation in the bla, 50 minutes after tbs stimulation
Exp Paradigm: Whole-cell patch clamp: current-clamp mode in pyramidal cells of the basolateral amygdala (bla); long term potentiation (ltp) using theta burst stimulation |
|
|
| Neurophysiology |
Miniature post synaptic current frequency: excitatory1 |
Increased
Description: Increased frequency of mepscs
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla)
|
Whole-cell patch clamp |
2-3 months |
| |
|
Description: Increased frequency of mepscs
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla) |
|
|
| Neurophysiology |
Spontaneous post synaptic events: inhibitory currents1 |
Increased
Description: Increased frequency of sipscs, but no change in amplitude
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla)
|
Whole-cell patch clamp |
2-3 months |
| |
|
Description: Increased frequency of sipscs, but no change in amplitude
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla) |
|
|
| Neurophysiology |
Spontaneous post synaptic events: excitatory currents1 |
Increased
Description: Increased frequency of sepscs, but no change in amplitude
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla)
|
Whole-cell patch clamp |
2-3 months |
| |
|
Description: Increased frequency of sepscs, but no change in amplitude
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla) |
|
|
| Neurophysiology |
Miniature post synaptic current frequency: inhibitory1 |
Increased
Description: Increased frequency of mipscs
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla)
|
Whole-cell patch clamp |
2-3 months |
| |
|
Description: Increased frequency of mipscs
Exp Paradigm: Whole-cell patch clamp: pyramidal neurons of the basolateral amygdala (bla) |
|
|
| Social behavior |
Social memory1 |
Decreased
Description: Decreased preference for novel mouse at 24-hour test, although short-term social memory (10-minute test) was not affected
Exp Paradigm: NA
|
Reciprocal social interaction test |
Adult |
| |
|
Description: Decreased preference for novel mouse at 24-hour test, although short-term social memory (10-minute test) was not affected
Exp Paradigm: NA |
|
|
| Developmental profile |
Developmental trajectory2 |
Increased
Description: Increased rate of tumorigenesis; neurofibrosarcoma, adrenal tumor, pheochromocytoma, myeloid leukemia
Exp Paradigm: Histopathalogical analysis
|
Histology |
27 months |
| |
|
Description: Increased rate of tumorigenesis; neurofibrosarcoma, adrenal tumor, pheochromocytoma, myeloid leukemia
Exp Paradigm: Histopathalogical analysis |
|
|
| Developmental profile |
Developmental trajectory2 |
Increased
Description: Increased rate of tumorigenesis especially onces linked with human disease: neurofibrosarcoma, adrenal tumor, pheochromocytoma, myeloid leukemia
Exp Paradigm: Histopathalogical analysis
|
Histology |
27 months |
| |
|
Description: Increased rate of tumorigenesis especially onces linked with human disease: neurofibrosarcoma, adrenal tumor, pheochromocytoma, myeloid leukemia
Exp Paradigm: Histopathalogical analysis |
|
|
| Molecular profile |
Protein expression level evidence2 |
Decreased
Description: Nf1 protein expression approximately halved
Exp Paradigm: Nf1 protein expression
|
NA |
Unreported |
| |
|
Description: Nf1 protein expression approximately halved
Exp Paradigm: Nf1 protein expression |
|
|
| Molecular profile |
Loss of heterozygosity (loh)2 |
Decreased
Description: Loss of wild-type allele
Exp Paradigm: Loss of heterozygosity (loh) analysis
|
Southern blot |
Unreported |
| |
|
Description: Loss of wild-type allele
Exp Paradigm: Loss of heterozygosity (loh) analysis |
|
|
| Molecular profile |
Protein expression level evidence1 |
Decreased
Description: Decreased number of adam22 immunoreactive cells in the bla, although no change in number of immunoreactive cells for cap1 or hsp70
Exp Paradigm: Immunohistochemistry: adam22 antibody
|
Immunohistochemistry |
Adult |
| |
|
Description: Decreased number of adam22 immunoreactive cells in the bla, although no change in number of immunoreactive cells for cap1 or hsp70
Exp Paradigm: Immunohistochemistry: adam22 antibody |
|
|
| Molecular profile |
Targeted expression2 |
Decreased
Description: Decreased nf1 protein expression
Exp Paradigm: Nf1 protein expression
|
Immunoprecipitation |
Unreported |
| |
|
Description: Decreased nf1 protein expression
Exp Paradigm: Nf1 protein expression |
|
|
| Molecular profile |
Protein expression level evidence1 |
Abnormal
Description: Altered expression of 73 proteins in neurons from basolateral amygdala, compared to wild type, out of 380 proteins assessed
Exp Paradigm: Liquid chromatography-mass spectrometry (lc-ms)
|
Liquid chromatography-mass spectrometry (lc-ms) |
Adult |
| |
|
Description: Altered expression of 73 proteins in neurons from basolateral amygdala, compared to wild type, out of 380 proteins assessed
Exp Paradigm: Liquid chromatography-mass spectrometry (lc-ms) |
|
|
| Molecular profile |
Protein phosphorylation1 |
Increased
Description: Increased number of immunoreactive phospho-erk cells in the basolateral amygdala (bla), compared to wild type, but not in the medial amygdala, 80 minutes after exposure to a novel mouse
Exp Paradigm: Immunohistochemistry: phospho-erk antibodies; reciprocal social interaction test with novel mouse
|
Immunohistochemistry |
Adult |
| |
|
Description: Increased number of immunoreactive phospho-erk cells in the basolateral amygdala (bla), compared to wild type, but not in the medial amygdala, 80 minutes after exposure to a novel mouse
Exp Paradigm: Immunohistochemistry: phospho-erk antibodies; reciprocal social interaction test with novel mouse |
|
|
| Molecular profile |
Loss of heterozygosity (loh)2 |
Decreased
Description: Loss of wild-type allele in dna from tumor
Exp Paradigm: Loss of heterozygosity (loh) analysis
|
Southern blot |
Unreported |
| |
|
Description: Loss of wild-type allele in dna from tumor
Exp Paradigm: Loss of heterozygosity (loh) analysis |
|
|
| Emotion |
Anxiety1 |
No change |
Elevated plus maze test |
Adult |
| Emotion |
Depression1 |
No change |
Forced swim test |
Adult |
| Learning & memory |
Cued or contextual fear conditioning: passive avoidance1 |
No change |
Passive avoidance test |
Adult |
| Learning & memory |
Object recognition memory1 |
No change |
Novel object recognition test |
Adult |
| Molecular profile |
Gene expression2 |
No change |
Northern blot |
Unreported |
| Molecular profile |
Gene expression2 |
No change |
Northern blot |
Unreported |
| Neurophysiology |
Miniature post synaptic current amplitude: excitatory1 |
No change |
Whole-cell patch clamp |
2-3 months |
| Neurophysiology |
Miniature post synaptic current amplitude: inhibitory1 |
No change |
Whole-cell patch clamp |
2-3 months |
| Sensory |
Olfaction1 |
No change |
Olfactory discrimination test |
Adult |
| Social behavior |
Social approach1 |
No change |
Reciprocal social interaction test |
Adult |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Emotion, Immune response, Learning & memory, Maternal behavior, Motor phenotype, Neuroanatomy / ultrastructure / cytoarchitecture, Neurophysiology, Physiological parameters, Repetitive behavior, Seizure, Sensory, Social behavior, |
