| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Circadian sleep/wake cycle |
Circadian rhythm: protein expression periodicity1 |
Decreased
Description: Nf1 mutant flies showed a decrease in PDF protein expression compared to controls.
Exp Paradigm: Protein expression measured at two time points (CT1 and CT13) in flies maintained in constant darkness (DD).
|
Immunohistochemistry |
Unreported |
| |
|
Description: Nf1 mutant flies showed a decrease in PDF protein expression compared to controls.
Exp Paradigm: Protein expression measured at two time points (CT1 and CT13) in flies maintained in constant darkness (DD). |
|
|
| Circadian sleep/wake cycle |
Circadian rhythm1 |
Decreased
Description: Nf1 mutant flies showed a dramatic decrease in rhythmicity (8.9% of flies were rhythmic) compared to controls.
|
General observations |
Unreported |
| |
|
Description: Nf1 mutant flies showed a dramatic decrease in rhythmicity (8.9% of flies were rhythmic) compared to controls.
|
|
|
| Neurophysiology |
Ion influx and permeability: calcium1 |
Abnormal
Description: Nf1 mutants showed do not show change or cycling in overall calcium levels compared to controls in light:dark cycles, that is observed in DH44 cells
Exp Paradigm: Calcium levels were measured at ZT1 and ZT13.
|
Calcium imaging |
Unreported |
| |
|
Description: Nf1 mutants showed do not show change or cycling in overall calcium levels compared to controls in light:dark cycles, that is observed in DH44 cells
Exp Paradigm: Calcium levels were measured at ZT1 and ZT13. |
|
|
| Neurophysiology |
Ion influx and permeability: calcium1 |
Increased
Description: Nf1 mutants showed a robust increase in calcium levels in SIFa-producing cells compared to controls.
|
Calcium imaging |
Unreported |
| |
|
Description: Nf1 mutants showed a robust increase in calcium levels in SIFa-producing cells compared to controls.
|
|
|
| Molecular profile |
Gene expression1 |
Increased
Description: Nf1 mutant flies showed an increase in SIF1 mRNA expression compared to controls.
|
Quantitative pcr (qrt-pcr) |
Unreported |
| |
|
Description: Nf1 mutant flies showed an increase in SIF1 mRNA expression compared to controls.
|
|
|
| Molecular profile |
Protein expression level evidence1 |
Decreased
Description: Nf1 mutant flies showed a decrease in SIFa protein expression compared to controls.
|
Immunohistochemistry |
Unreported |
| |
|
Description: Nf1 mutant flies showed a decrease in SIFa protein expression compared to controls.
|
|
|
| Circadian sleep/wake cycle |
Circadian rhythm1 |
No change |
Calcium imaging |
Unreported |
| Circadian sleep/wake cycle |
Circadian rhythm: protein expression periodicity1 |
No change |
Immunohistochemistry |
Unreported |
| Molecular profile |
Protein expression level evidence1 |
No change |
Immunohistochemistry |
Unreported |
|
| Not Reported: |
Communications, Developmental profile, Emotion, Immune response, Learning & memory, Maternal behavior, Motor phenotype, Neuroanatomy / ultrastructure / cytoarchitecture, Physiological parameters, Repetitive behavior, Seizure, Sensory, Social behavior, |
