| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
General locomotor activity: ambulatory activity1 |
Ameliorated
Description: Increased expression of gabrb3 in mutants show increase in total distance travelled in mutants.
Exp Paradigm: NA
|
Open field test |
6 weeks |
| |
|
Description: Increased expression of gabrb3 in mutants show increase in total distance travelled in mutants.
Exp Paradigm: NA |
|
|
| Motor phenotype |
General locomotor activity1 |
No adverse effect
Description: Increased expression of gabrb3 in mutants show no change in total arm entries in the elevated plus maze in mutants.
Exp Paradigm: NA
|
Elevated plus maze test |
6 weeks |
| |
|
Description: Increased expression of gabrb3 in mutants show no change in total arm entries in the elevated plus maze in mutants.
Exp Paradigm: NA |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Neuronal number: interneurons1 |
Ameliorated
Description: Increased expression of gabrb3 decreased parvalbumin positive neurons in the primary somatosensory cortex, and in the basolateral amygdala and had no adverse effect in the visual cortex in mutants.
Exp Paradigm: NA
|
Immunohistochemistry |
6 weeks |
| |
|
Description: Increased expression of gabrb3 decreased parvalbumin positive neurons in the primary somatosensory cortex, and in the basolateral amygdala and had no adverse effect in the visual cortex in mutants.
Exp Paradigm: NA |
|
|
| Neuroanatomy / Ultrastructure / Cytoarchitecture |
Neuroreceptor levels: gaba-r: gabaa1 |
Ameliorated
Description: Gabrb3-mcherry plasmid injected mutants expressed mcherry in an average of 30% drg neurons and an average of 30% nf200 drg neurons. mutants injected with aav.flex.gabrb3.mcherry show decreased expression of gabrb3 expression in the spinal cord dorsal horn lamina iii/iv at vglut1 positive presynaptic terminals for abeta and adelta ltmrs compared with controls but increased compared with m_mecp2_57_ki_he.
Exp Paradigm: Spinal cord
|
Immunohistochemistry |
6 weeks |
| |
|
Description: Gabrb3-mcherry plasmid injected mutants expressed mcherry in an average of 30% drg neurons and an average of 30% nf200 drg neurons. mutants injected with aav.flex.gabrb3.mcherry show decreased expression of gabrb3 expression in the spinal cord dorsal horn lamina iii/iv at vglut1 positive presynaptic terminals for abeta and adelta ltmrs compared with controls but increased compared with m_mecp2_57_ki_he.
Exp Paradigm: Spinal cord |
|
|
| Sensory |
Tactile memory1 |
Restored
Description: Increased expression of gabrb3 increased preference for object with a novel texture in mutants to control levels.
Exp Paradigm: NA
|
Textured novel object recognition test (t-nort) |
6 weeks |
| |
|
Description: Increased expression of gabrb3 increased preference for object with a novel texture in mutants to control levels.
Exp Paradigm: NA |
|
|
| Sensory |
Sensorimotor gating: tactile cue1 |
Refractory
Description: Increased expression of gabrb3 in mutants show no change in percent prepulse inhibition of the startle response to a 125 db noise, when the startle noise is preceded by a light air puff, in mutants.
Exp Paradigm: NA
|
Prepulse inhibition |
6 weeks |
| |
|
Description: Increased expression of gabrb3 in mutants show no change in percent prepulse inhibition of the startle response to a 125 db noise, when the startle noise is preceded by a light air puff, in mutants.
Exp Paradigm: NA |
|
|
| Sensory |
Startle response: acoustic stimulus1 |
Refractory
Description: Increased expression of gabrb3 in mutants show no change in amplitude of startle response to a 125 db startling noise and to a 80db non-startling noise pulse, in mutants.
Exp Paradigm: NA
|
Acoustic startle reflex test |
6 weeks |
| |
|
Description: Increased expression of gabrb3 in mutants show no change in amplitude of startle response to a 125 db startling noise and to a 80db non-startling noise pulse, in mutants.
Exp Paradigm: NA |
|
|
| Sensory |
Sensorimotor gating: tactile cue1 |
Ameliorated
Description: Increased expression of gabrb3 decreased the startle response to an air puff alone in mutants.
Exp Paradigm: NA
|
Response to air puff |
6 weeks |
| |
|
Description: Increased expression of gabrb3 decreased the startle response to an air puff alone in mutants.
Exp Paradigm: NA |
|
|
| Sensory |
Sensorimotor gating: tactile cue1 |
Ameliorated
Description: Increased expression of gabrb3 in somatosensory neurons decreased percent inhibition of the startle response to a 125 db noise, when the startle noise is preceded by a light air puff, in mutants
Exp Paradigm: NA
|
Prepulse inhibition |
6 weeks |
| |
|
Description: Increased expression of gabrb3 in somatosensory neurons decreased percent inhibition of the startle response to a 125 db noise, when the startle noise is preceded by a light air puff, in mutants
Exp Paradigm: NA |
|
|
| Sensory |
Sensorimotor gating1 |
Refractory
Description: Increased expression of gabrb3 in mutants shows no change in percent inhibition of the startle response to a 125 db noise pulse when the startle noise is preceded by an 80 db tone prepulse, in mutants.
Exp Paradigm: NA
|
Prepulse inhibition |
6 weeks |
| |
|
Description: Increased expression of gabrb3 in mutants shows no change in percent inhibition of the startle response to a 125 db noise pulse when the startle noise is preceded by an 80 db tone prepulse, in mutants.
Exp Paradigm: NA |
|
|
| Sensory |
Sensorimotor gating: tactile cue1 |
Refractory
Description: Increased expression of gabrb3 in mutants show no change in responsivity to an air puff stimulus delivered to back hairy skin in mutants.
Exp Paradigm: NA
|
Response to air puff |
6 weeks |
| |
|
Description: Increased expression of gabrb3 in mutants show no change in responsivity to an air puff stimulus delivered to back hairy skin in mutants.
Exp Paradigm: NA |
|
|
| Social behavior |
Social memory1 |
Restored
Description: Increased expression of gabrb3 increased preference for novel mouse in mutants.
Exp Paradigm: NA
|
Three-chamber social approach test |
6 weeks |
| |
|
Description: Increased expression of gabrb3 increased preference for novel mouse in mutants.
Exp Paradigm: NA |
|
|
| Social behavior |
Social approach1 |
Restored
Description: Increased expression of gabrb3 increased preference for novel mouse in mutants.
Exp Paradigm: NA
|
Three-chamber social approach test |
6 weeks |
| |
|
Description: Increased expression of gabrb3 increased preference for novel mouse in mutants.
Exp Paradigm: NA |
|
|
| Emotion |
Anxiety1 |
Ameliorated
Description: Increased expression of gabrb3 increased time spent in the center in the mutants.
Exp Paradigm: NA
|
Open field test |
6 weeks |
| |
|
Description: Increased expression of gabrb3 increased time spent in the center in the mutants.
Exp Paradigm: NA |
|
|
| Emotion |
Habituation to aversive stimuli1 |
Restored
Description: Increased expression of gabrb3 in mutants increased startle amplitude to a 125 db noise during a 30-minute tactile ppi session, when comparing the first five startle responses to the last five
Exp Paradigm: NA
|
NA |
NA |
| |
|
Description: Increased expression of gabrb3 in mutants increased startle amplitude to a 125 db noise during a 30-minute tactile ppi session, when comparing the first five startle responses to the last five
Exp Paradigm: NA |
|
|
| Emotion |
Exploratory activity1 |
No adverse effect
Description: Increased expression of gabrb3 in mutants show no change in the time spent investigating objects in mutants.
Exp Paradigm: NA
|
Novel object recognition test |
6 weeks |
| |
|
Description: Increased expression of gabrb3 in mutants show no change in the time spent investigating objects in mutants.
Exp Paradigm: NA |
|
|
| Emotion |
Anxiety1 |
Restored
Description: Increased expression of gabrb3 increased time spent in the open arms in mutants.
Exp Paradigm: NA
|
Elevated plus maze test |
6 weeks |
| |
|
Description: Increased expression of gabrb3 increased time spent in the open arms in mutants.
Exp Paradigm: NA |
|
|
| Learning & memory |
Object recognition memory: long-term recall1 |
Refractory
Description: Increased expression of gabrb3 in mutants showed no change in preference for novel textured object after 5 min or 1hr retention.
Exp Paradigm: NA
|
Textured novel object recognition test (t-nort) |
6 weeks |
| |
|
Description: Increased expression of gabrb3 in mutants showed no change in preference for novel textured object after 5 min or 1hr retention.
Exp Paradigm: NA |
|
|
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Developmental profile, Immune response, Maternal behavior, Molecular profile, Neurophysiology, Physiological parameters, Repetitive behavior, Seizure, |
