|
|
|
|
Animal Model >> null >> Z_NBEA_1_KO_HM Construct Details |
| Model Details :Z_NBEA_1_KO_HM
|
| |
|
Model Type |
Genetic
|
|
Model Genotype |
Homozygous
|
|
Mutation |
An ethylnitrosourea induced zebrafish mutant, called Disconnect4 (dis4), that was mapped to have 1 nonsense mutation in the neurobeachin gene that truncates the gene product at amino acid 906 along with 11 missense mutations in other genes. The nonsense mutation in nbea was demonstrated to be the mutation responsible for the dis4 phenotype as zebrafish with a 7 base pair (bp) frameshifting deletion in the first exon (nbea-fh392) and an 8 bp frameshifting deletion in exon 21 (nbea-fh380), generated using TALEN, failed to complement the dis4 ENU generated mutant phenotype. Based on mapping studies dis4 was renamed nbea-fh364. nbea-fh364 zebrafish has a C to T transition in exon 21 introducing a nonsense codon that truncates the protein approximately one third of the way through the protein. Et(Tol-056:GFP) transgenic line which expresses GFP in Mauthner and Commissural Local neurons was used to identify these neuron types. The genotype of the fish is: nbea^fh364/fh364; zf206Et (AB/TU). |
|
Allele Type |
Targeted (knockout) |
|
Strain of Origin |
|
|
Genetic Background |
|
|
ES Cell Line |
|
|
Mutant ES Cell Line |
|
|
Model Source |
Walker C, 2009 (PMID:19568220) |
|
Reference |
Miller AC , et al. 2014
|
|
|
