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Animal Model >> null >> M_NLGN3_1_KI_R451C_GEPH-GFP Construct Details |
| Model Details :M_NLGN3_1_KI_R451C_GEPH-GFP
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Model Type |
Genetic
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Model Genotype |
Hemizygous
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Mutation |
The progenitor cells for layer 2/3 neurons in the somatosensory cortex and anterior frontal cortex of NLGN3_1_KI_R451C mice were transfected using in utero electroporation to label excitatory synapses. E15.5 timed pregnant mice were deeply anaesthetized, their uterine horns were exposed and approximately 1ul of DNA mixture, containing DsRed2 plasmid (1ug/ul) and Gephyrin-GFP plasmid (1-2ug/ul), was pressure injected into the lateral ventricle of each embryo through a micropipette. Electric pulses were passed through the head of each embryo using two tweezer type electrodes for electroporation. |
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Allele Type |
ASD LOF mutation |
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Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+ |
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Genetic Background |
129S1/Sv * 129X1/SvJ * C57BL/6 |
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ES Cell Line |
R1 |
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Mutant ES Cell Line |
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Model Source |
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Reference |
Isshiki M , et al. 2014
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