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Animal Model >> null >> M_MECP2_57_KI_HE_GABRB3-CRE Construct Details |
| Model Details :M_MECP2_57_KI_HE_GABRB3-CRE
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Model Type |
RESCUE-Genetic
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Model Genotype |
Hemizygous
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Mutation |
Male knockin mice harboring an arginine-to-cysteine missense R306 mutation in Mecp2 (Mecp2^R306C), a common allele in Rett syndrome patients together with Advillin-Cre (Advillin-Cre; Mecp2^C/y). Male Advillin-Cre; Mecp2^C/y mice were intraperitoneally injected at P5 with AAV.FLEx.GABRB3.mCHERRY to restore GABRB3 expression selectively in peripheral somatosensory neurons. Mecp2^R306C littermate mice lacking the Advillin-Cre allele and injected with AAV.FLEx.GABRB3.mCHERRY served as a control together with wild-type littermates with and without the Advillin-Cre allele. Intraperitoneal injection of Advillin-Cre; Mecp2^R306C mice, but not Mecp2^R306C mice lacking the Advillin-Cre allele, with AAV.FLEx.GABRB3.mCHERRY led to transduction of all types of DRG sensory neurons. Wildtype controls were Mecp2^R/y male littermates injected with AAV.FLEx.GABRB3.mCHERRY. |
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Allele Type |
ASD LOF knockin |
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Strain of Origin |
Not specified |
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Genetic Background |
C57BL/6J |
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ES Cell Line |
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Mutant ES Cell Line |
129/Ola E14 TG2a mouse ES cells |
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Model Source |
Michael Greenberg; Lyst et al., 2013; PMID 23770565 |
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Reference |
Orefice LL , et al. 2019
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