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Animal Model >> null >> M_MECP2_39_KI_HE_R306C Construct Details |
| Model Details :M_MECP2_39_KI_HE_R306C
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Model Type |
Genetic
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Model Genotype |
Hemizygous
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Mutation |
The point mutation R306C found in 5% of Rhett syndrome patients, was introduced in a Mecp2-EGFP targeting vector containing exon3 and part of exon4 followed by a loxP flanked neoSTOP cassette as a selectable marker; linearized vector was electroporated into 129/OlaE14 TG2a ES cells and chimeric mice generated by standard procedures. The loxP flanked neo cassette was removed by crossing to pCAGGS-cre mice, In the article by Orefice et al it is unclear if the EGFP tagged or tag-removed Mecp2 R306C mice were used. |
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Allele Type |
LOF knockin |
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Strain of Origin |
129 Ola |
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Genetic Background |
C57BL/6 |
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ES Cell Line |
129/Ola E14 TG2a |
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Mutant ES Cell Line |
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Model Source |
PMID 23770565 |
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Reference |
Orefice LL , et al. 2016
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