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Animal Model >> null >> M_MECP2_36.1_TG_7X_ASO Construct Details |
| Model Details :M_MECP2_36.1_TG_7X_ASO
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Model Type |
RESCUE-Genetic
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Model Genotype |
Transgenic
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Mutation |
F1 hybrid animals generated by mating FVB/N MECP2-TG1 females and wild-type 129S6/SvEv male mice (Taconic Farms) were treated with antisense oligonucleotide 5 (ASO5) designed to anneal at the intron between exons 2 and 3 of the human MECP2 pre-mRNA; gradually infused ASO into the brains of 78-week-old mice using micro-osmotic pumps designed to deliver the molecule at a constant rate over a 4-week period; ASOs designed to bind several regions of the human MECP2 precursor mRNA (pre-mRNA) so as to reduce the levels of both alternatively spliced MECP2 isoforms, MECP2-e1 and MECP2-e2;. |
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Allele Type |
Conditional knockout |
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Strain of Origin |
FVB/N |
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Genetic Background |
FVB/N x 129S6/SvEv |
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ES Cell Line |
Not Specified |
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Mutant ES Cell Line |
Not Specified |
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Model Source |
Taconic Farms |
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Reference |
Sztainberg Y , et al. 2015
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