Slc16a8
Mus musculus
Gene Name: solute carrier family 16, member 8 (monocarboxylic acid transporter 3)
Aliases: Mct3; MCT 3; monocarboxylate transporter 3; preferentially expressed in RPE cells and choroid plexus epithelium; proton-coupled monocarboxylate transporter 3; solute carrier family 16 member 8
Human Ortholog: SLC16A8
Aliases: Mct3; MCT 3; monocarboxylate transporter 3; preferentially expressed in RPE cells and choroid plexus epithelium; proton-coupled monocarboxylate transporter 3; solute carrier family 16 member 8
Human Ortholog: SLC16A8
Summary Statistics:
# of Reports: 1
# of Models: 1
# of Reports: 1
# of Models: 1
External Links
Model Summary
Disruption of Slc16a8 leads to a potentially reversible decrease in subretinal space pH, thereby reducing the magnitude of the light suppressible photoreceptor current.
References
Type
Title
Author, Year
Primary
Altered visual function in monocarboxylate transporter 3 (Slc16a8) knockout mice.
M_SLC16A8_1_KO_HM
Model Type:
Genetic
Model Genotype: Homozygous
Mutation: Targeted disruption of 2.2 kb of DNA spanning the entire coding portion of exons 2-4 of Slc16a8 gene.
Allele Type: Targeted (knockout)
Strain of Origin: Not specified
Genetic Background: C57B1/6J
ES Cell Line: Not specified
Mutant ES Cell Line: Not specified
Model Source: Not specified
Model Genotype: Homozygous
Mutation: Targeted disruption of 2.2 kb of DNA spanning the entire coding portion of exons 2-4 of Slc16a8 gene.
Allele Type: Targeted (knockout)
Strain of Origin: Not specified
Genetic Background: C57B1/6J
ES Cell Line: Not specified
Mutant ES Cell Line: Not specified
Model Source: Not specified
M_SLC16A8_1_KO_HM
Category
Entity
Quantity
Experimental Paradigm
Age at Testing
Electroretinogram (erg)1
Decreased
Description: Decreased erg response indicated by reduced magnitude of photoreceptor-derived a-wave and bipolar cell-derived b-waves
Exp Paradigm: Electroretinogram record
Exp Paradigm: Electroretinogram record
Electroretinogram (erg)
9 weeks
Protein expression level evidence1
Increased
Description: Increased expression of mct1 in rpe
Exp Paradigm: Mct1 protein expression
Exp Paradigm: Mct1 protein expression
Western blot
3-4 months
Gene expression1
Decreased
Description: Decreased expression of slc16a8 in basolateral membrane of rpe
Exp Paradigm: Slc16a8 expression
Exp Paradigm: Slc16a8 expression
Immunohistochemistry
3 months
Protein expression level evidence1
Decreased
Description: Decreased mrna and protein expression of slc16a8 in rpe
Exp Paradigm: Slc16a8 mrna/protein expression-semi-quantitative pcr (qrt-pcr)
Exp Paradigm: Slc16a8 mrna/protein expression-semi-quantitative pcr (qrt-pcr)
Semi-quantitative pcr (qrt-pcr)
Unreported
Protein expression level evidence1
Decreased
Description: Decreased mrna and protein expression of slc16a8 in rpe
Exp Paradigm: Slc16a8 mrna/protein expression- western blot
Exp Paradigm: Slc16a8 mrna/protein expression- western blot
Western blot
Unreported
Protein expression level evidence1
Increased
Description: Increased lactate concentration in retinal washes
Exp Paradigm: Lactate expression
Exp Paradigm: Lactate expression
Enzymatic measurement of lactate in retinas
3-4 months
Protein expression level evidence1
Abnormal
Description: Abnormal cd147 expression in the apical membrane of rpe
Exp Paradigm: Cd147 expression
Exp Paradigm: Cd147 expression
Immunohistochemistry
3 months
Not Reported:
Circadian sleep/wake cycle, Communications, Emotion, Immune response, Learning & memory, Maternal behavior, Motor phenotype, Neuroanatomy / ultrastructure / cytoarchitecture, Physiological parameters, Repetitive behavior, Seizure, Social behavior