Rpl10 suppression, mediated by translation and splice blocking morpholinos in zebrafish, results in microcephaly concomitant with reduced bulk translation and increased apoptosis in the brain although total body length is not affected.
References
Type
Title
Author, Year
Primary
A novel ribosomopathy caused by dysfunction of RPL10 disrupts neurodevelopment and causes X-linked microcephaly in humans.
Model Type:
Genetic
Model Genotype:
Wild type
Mutation:
Zebrafish morphants with rpl10 gene suppression using a translation blocking morpholino targeting the translational start site of the rpl10 transcript injected into 1-4 cell stage embryos at a dose of 0.5 ng. .
Allele Type: Knockdown
Strain of Origin: Genetic Background: ES Cell Line: Mutant ES Cell Line: Model Source:
Model Type:
Genetic
Model Genotype:
Wild type
Mutation:
Zebrafish morphants with rpl10 gene suppression using a translation blocking morpholino targeting the translational start site of the rpl10 transcript injected into 1-4 cell stage embryos at a dose of 0.6 ng. .
Allele Type: Knockdown
Strain of Origin: Genetic Background: ES Cell Line: Mutant ES Cell Line: Model Source:
Model Type:
Genetic
Model Genotype:
Wild type
Mutation:
Zebrafish morphants with rpl10 gene suppression using a translation blocking morpholino targeting the translational start site of the rpl10 transcript injected into 1-4 cell stage embryos at a dose of 0.7 ng. .
Allele Type: Knockdown
Strain of Origin: Genetic Background: ES Cell Line: Mutant ES Cell Line: Model Source:
Model Type:
Genetic
Model Genotype:
Wild type
Mutation:
Zebrafish morphants with rpl10 gene suppression using a splice blocking morpholino that results in skipping of exon 2 encoding a premature stop codon injected into 1-4 cell stage embryos at a dose of 1 ng.
Allele Type: Knockdown
Strain of Origin: Genetic Background: ES Cell Line: Mutant ES Cell Line: Model Source:
Model Type:
Genetic
Model Genotype:
Wild type
Mutation:
Zebrafish morphants with rpl10 gene suppression using a splice blocking morpholino that results in skipping of exon 2 encoding a premature stop codon injected into 1-4 cell stage embryos at a dose of 2 ng.
Allele Type: Knockdown
Strain of Origin: Genetic Background: ES Cell Line: Mutant ES Cell Line: Model Source:
Model Type:
Genetic
Model Genotype:
Wild type
Mutation:
Zebrafish morphants with rpl10 gene suppression using a splice blocking morpholino that results in skipping of exon 2 encoding a premature stop codon injected into 1-4 cell stage embryos at a dose of 3 ng.
Allele Type: Knockdown
Strain of Origin: Genetic Background: ES Cell Line: Mutant ES Cell Line: Model Source:
Description: Morphants show decreased head size compared to controls.
Exp Paradigm: An outline was drawn beginning at the posteriormost point of eye and tracing around the head to terminate at the starting point to measure forebrain cross sectional area.
Description: Morphants show increased apoptosis in the brain compared to controls.
Exp Paradigm: 20 embryos of morphants and controls each were used for TUNEL assay.
Description: Morphants show decreased head size compared to controls.
Exp Paradigm: An outline was drawn beginning at the posteriormost point of eye and tracing around the head to terminate at the starting point to measure forebrain cross sectional area.
Description: Morphants show increased apoptosis in the brain compared to controls.
Exp Paradigm: 20 embryos of morphants and controls each were used for TUNEL assay.
Description: Morphants show decreased head size compared to controls.
Exp Paradigm: An outline was drawn beginning at the posteriormost point of eye and tracing around the head to terminate at the starting point to measure forebrain cross sectional area.
Description: Morphants show increased apoptosis in the brain compared to controls.
Exp Paradigm: 20 embryos of morphants and controls each were used for TUNEL assay.
Description: Morphants show decreased head size compared to controls.
Exp Paradigm: An outline was drawn beginning at the posteriormost point of eye and tracing around the head to terminate at the starting point to measure forebrain cross sectional area.
Description: Morphants show increased apoptosis in the brain compared to controls.
Exp Paradigm: 20 embryos of morphants and controls each were used for TUNEL assay.
Description: Morphants show decreased translational activity in the head compared to controls.
Exp Paradigm: Increase in 80S ribosomal subunit and a decrease in polyribosome was used to measure translational activity.
Description: Morphants show decreased head size compared to controls.
Exp Paradigm: An outline was drawn beginning at the posteriormost point of eye and tracing around the head to terminate at the starting point to measure forebrain cross sectional area.
Description: Morphants show increased apoptosis in the brain compared to controls.
Exp Paradigm: 20 embryos of morphants and controls each were used for TUNEL assay.
Description: Morphants show decreased translational activity in the head compared to controls.
Exp Paradigm: Increase in 80S ribosomal subunit and a decrease in polyribosome was used to measure translational activity.
Description: Morphants show decreased head size compared to controls.
Exp Paradigm: An outline was drawn beginning at the posteriormost point of eye and tracing around the head to terminate at the starting point to measure forebrain cross sectional area.
Description: Morphants show increased apoptosis in the brain compared to controls.
Exp Paradigm: 20 embryos of morphants and controls each were used for TUNEL assay.
Description: Morphants show decreased translational activity in the head compared to controls.
Exp Paradigm: Increase in 80S ribosomal subunit and a decrease in polyribosome was used to measure translational activity.
Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro
Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites
