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No Human Gene Data Available
Chromosome
CNV Locus
CNV Type
# of studies
Animal Model
17
Deletion-Duplication
 29
 
17
Duplication
 2
 
17
Duplication
 8
 
17
Duplication
 1
 
17
Duplication
 3
 
17
Duplication
 1
 

Model Summary

Regulation of thymocyte development, T cell activation, neutrophil migratin, oxidative burst.

References

Type
Title
Author, Year
Primary
Function of PI3Kgamma in thymocyte development, T cell activation, and neutrophil migration.

M_PIK3R6_1_KO_HM

Model Type: Genetic
Model Genotype: Homozygous
Mutation: Gene targeted disruption of the p110 catalytic PI3K subunit.
Allele Type: Targeted (Knock Out)
Strain of Origin: Not Specified
Genetic Background: 129/J
ES Cell Line: E14K
Mutant ES Cell Line: Not Specified
Model Source: Not Specified

M_PIK3R6_2_KO_HT

Model Type: Genetic
Model Genotype: Heterozygous
Mutation: Gene targeted disruption of the p110 catalytic PI3K subunit.
Allele Type: Targeted (Knock Out)
Strain of Origin: Not Specified
Genetic Background: 129/J
ES Cell Line: E14K
Mutant ES Cell Line: Not Specified
Model Source: Not Specified

M_PIK3R6_1_KO_HM

Category
Entity
Quantity
Experimental Paradigm
Age at Testing
Immune cell signaling1
Abnormal
Description: Decreased phosphorylation of erk1 and erk2, pkb, type-ia pi3k by thrombin; no change in scf-stimulatino of type-ia pi3k in bone marrow mast cells (bmmc)
Exp Paradigm: Western blot analysis using phospho-specific antibodies against pkb, erk1 and erk2, and s6k and thin-layer chromatography after stimulation with thrombin (thr, 5 units/ml), sct (100ng/ml), and il-3 of serum-starved bmmcs
 Western blot
 Unreported
Innate immune response: neutrophil migration1
Decreased
Description: Decreased accumulation of neutrophils in peritoneal cavities in both casein- and listeria monocytogenes; decreased chemotaxis of neutrophils in response to fmlp- and c5a
Exp Paradigm: Numeration of neutrophils in response to induced peritonitis; quantification of chemotaxic movement- in vitro neutrophil chemotaxis: fmlp/ c5a
 In vitro neutrophil chemotaxis
 Unreported
Development of immune cells and primary lymphoid organs1
Decreased
Description: Decreased thymic cellularity; decreased proportion of double positive (dp) cells; increase in double negative (dn) cells; decreased thymocyte survival, specifically dp cells after stimulation with adenosine receptor agonist
Exp Paradigm: Flow cytometric analysis
 Flow cytometric analysis
 Unreported
Immune cell signaling1
Decreased
Description: Decreased respiratory burst induction by protein kinase c-activator phorbol 12-myrisate 13-acetate (pma) in bone marrow neutrophils
Exp Paradigm: Spectroscopical analysis of oxygen radical production in response to fmlp (8 m), c5a (100 ng/ml); pma (0.5 m)
 Spectroscopy
 Unreported
Adaptive immune response: t cells1
Decreased
Description: Reduced lcmv-induced footpad-swelling; no change in primary cytotoxic response to lcmv; reduced nip-specific igg1 antibody titers
Exp Paradigm: Footpad-swelling reaction: inoculation with 2000 pfu of lcmv, and swelling assessed daily
 Local inflammatory reaction
 Unreported
Immune cell signaling1
Decreased
Description: Decreased ptdins (3,4,5)p3 accumulation; decreased phosphorylation of pkb, s6k, and erk1 and erk2; no change in p38 phosphorylation and overall tyrosine phosphorylation in neutrophils
Exp Paradigm: Western blot analysis using phospho-specific antibodies against pkb, erk1 and erk2, and s6k and thin-layer chromatography after stimulation with fmlp (8 m) or c5a (100ng/ml), with or without pre-treatment with wortmaniin (100 nm)
 Western blot
 Unreported
Adaptive immune response: t cells1
Decreased
Description: Decreased proliferation of t cells in response to anti-cd3 and con a; decreased amount of il-2 and ifn- cytokine secretion
Exp Paradigm: Elisa analysis
 Elisa
 Unreported
Innate immune response: neutrophil migration1
Decreased
Description: Decreased accumulation of neutrophils in peritoneal cavities in both casein- and listeria monocytogenes; decreased chemotaxis of neutrophils in response to fmlp- and c5a
Exp Paradigm: Numeration of neutrophils in response to induced peritonitis; quantification of chemotaxic movement-in vivo neutrophil migration: induced peritonitis
 In vivo neutrophil migration
 Unreported
General characteristics1
 No change
 General observations
 Unreported
Adaptive immune response: b cells1
 No change
 Elisa
 Unreported
Innate immune response1
 No change
 NA
 7-12 weeks
 Not Reported: Circadian sleep/wake cycle, Communications, Emotion, Learning & memory, Maternal behavior, Molecular profile, Motor phenotype, Neuroanatomy / ultrastructure / cytoarchitecture, Neurophysiology, Physiological parameters, Repetitive behavior, Seizure, Sensory, Social behavior

M_PIK3R6_2_KO_HT

Category
Entity
Quantity
Experimental Paradigm
Age at Testing
General characteristics1
 No change
 General observations
 Unreported
Adaptive immune response: b cells1
 No change
 Elisa
 Unreported
Adaptive immune response: t cells1
 No change
 Local inflammatory reaction
 Unreported
Adaptive immune response: t cells1
 No change
 Elisa
 Unreported
Development of immune cells and primary lymphoid organs1
 No change
 Flow cytometric analysis
 Unreported
Immune cell signaling1
 No change
 Western blot
 Unreported
Immune cell signaling1
 No change
 Western blot
 Unreported
Immune cell signaling1
 No change
 Spectroscopy
 Unreported
Innate immune response1
 No change
 NA
 7-12 weeks
Innate immune response: neutrophil migration1
 No change
 In vitro neutrophil chemotaxis
 Unreported
Innate immune response: neutrophil migration1
 No change
 In vivo neutrophil migration
 Unreported
 Not Reported: Circadian sleep/wake cycle, Communications, Emotion, Learning & memory, Maternal behavior, Molecular profile, Motor phenotype, Neuroanatomy / ultrastructure / cytoarchitecture, Neurophysiology, Physiological parameters, Repetitive behavior, Seizure, Sensory, Social behavior

No PIN Data Available
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