| Phenotypic Profile |
| Category |
Entity |
Quantity |
Experimental Paradigm |
Age at Testing |
| Motor phenotype |
Motor coordination and balance1 |
Abnormal
Description: Mecp2 tg (2x) mice failed to move in the dowel test and remained immobile for long periods of time
Exp Paradigm: NA
|
Dowel walk test |
6 weeks |
| |
|
Description: Mecp2 tg (2x) mice failed to move in the dowel test and remained immobile for long periods of time
Exp Paradigm: NA |
|
|
| Motor phenotype |
Motor coordination and balance1 |
Increased
Description: At 10 weeks and 20 weeks of age mecp2 tg (2x) mice has increased latency to fall off the accelerating rotarod compared to wildtype over 4 days of testing with 4 trials on each day, authors note that this may indicate enhanced cerebellar learning
Exp Paradigm: NA
|
Accelerating rotarod test |
10 weeks, 20 weeks |
| |
|
Description: At 10 weeks and 20 weeks of age mecp2 tg (2x) mice has increased latency to fall off the accelerating rotarod compared to wildtype over 4 days of testing with 4 trials on each day, authors note that this may indicate enhanced cerebellar learning
Exp Paradigm: NA |
|
|
| Motor phenotype |
Clasping reflex1 |
Increased
Description: Forepaw clasping in increased in mecp2 tg mice, first observed at 12-15 weeks
Exp Paradigm: NA
|
Tail suspension test |
12-15 weeks |
| |
|
Description: Forepaw clasping in increased in mecp2 tg mice, first observed at 12-15 weeks
Exp Paradigm: NA |
|
|
| Neurophysiology |
Electroencephalogram (eeg) signature1 |
Abnormal
Description: Recordings made in the absence of an observable clonic seizures in 20 week old mecp2 tg mice showes abnormal spikes
Exp Paradigm: NA
|
Electroencephalogram (eeg) |
20 weeks |
| |
|
Description: Recordings made in the absence of an observable clonic seizures in 20 week old mecp2 tg mice showes abnormal spikes
Exp Paradigm: NA |
|
|
| Neurophysiology |
Synaptic plasticity: hippocampal ltp1 |
Increased
Description: Ltp is also enhanced relative to controls in the hippocampal schaffer collateral -ca1 synapses in mecp2 tg(2x) mice
Exp Paradigm: NA
|
High-frequency stimulation (hfs) |
NA |
| |
|
Description: Ltp is also enhanced relative to controls in the hippocampal schaffer collateral -ca1 synapses in mecp2 tg(2x) mice
Exp Paradigm: NA |
|
|
| Neurophysiology |
Presynaptic function: paired-pulse facilitation1 |
Increased
Description: Ppf is greatly enhanced in hippocampal schaffer-collateral synapses in mecp2 tg mice
Exp Paradigm: NA
|
Whole-cell patch clamp |
NA |
| |
|
Description: Ppf is greatly enhanced in hippocampal schaffer-collateral synapses in mecp2 tg mice
Exp Paradigm: NA |
|
|
| Seizure |
Seizures1 |
Increased
Description: Increased clonic seizures are observed at 20 and 50 weeks of age for mecp2 tg (2x) mice , the seizures begin to manifest at 12-15 weeks
Exp Paradigm: NA
|
General observations |
12-15 weeks, 20 weeks, 50 weeks |
| |
|
Description: Increased clonic seizures are observed at 20 and 50 weeks of age for mecp2 tg (2x) mice , the seizures begin to manifest at 12-15 weeks
Exp Paradigm: NA |
|
|
| Seizure |
Electroencephalogram (eeg): signature of seizure/epilepsy1 |
Abnormal
Description: Recordings made during akinetic seizures (behavioral immobility) were also abnormal in 50 week olf mecp2 tg mice
Exp Paradigm: NA
|
Electroencephalogram (eeg) |
50 weeks |
| |
|
Description: Recordings made during akinetic seizures (behavioral immobility) were also abnormal in 50 week olf mecp2 tg mice
Exp Paradigm: NA |
|
|
| Sensory |
Vision1 |
Decreased
Description: The original human mecp2-overexpressing mice, mecp2-tg mice on a fvb/n pure background, develop premature retinal degeneration, which can confound the interpretation of some behavioural tests
Exp Paradigm: Ophthalmological evaluation;
|
Ophthalmological evaluation |
Adult |
| |
|
Description: The original human mecp2-overexpressing mice, mecp2-tg mice on a fvb/n pure background, develop premature retinal degeneration, which can confound the interpretation of some behavioural tests
Exp Paradigm: Ophthalmological evaluation; |
|
|
| Physiological parameters |
Reproductive function1 |
Decreased
Description: Mecp2 tg mice show decreased fertility
Exp Paradigm: NA
|
General observations |
12-15 weeks |
| |
|
Description: Mecp2 tg mice show decreased fertility
Exp Paradigm: NA |
|
|
| Developmental profile |
Skeletal development1 |
Decreased
Description: Increased observation of kyphosis ( outward curvature of spine) starting at age 12-15 weeks
Exp Paradigm: NA
|
General observations |
12-15 weeks |
| |
|
Description: Increased observation of kyphosis ( outward curvature of spine) starting at age 12-15 weeks
Exp Paradigm: NA |
|
|
| Developmental profile |
Mortality/lethality1 |
Increased
Description: Premature death is observed, starting with reduced survival at ~ 20 weeks of age in mecp2 tg(2x) mice
Exp Paradigm: NA
|
General observations |
18 weeks |
| |
|
Description: Premature death is observed, starting with reduced survival at ~ 20 weeks of age in mecp2 tg(2x) mice
Exp Paradigm: NA |
|
|
| Learning & memory |
Cued or contextual fear conditioning: memory of context1 |
Increased
Description: Mecp2 tg mice show increased freezing in response to context compared to wildtype controls, authors indicate enhanced hippocampal learning
Exp Paradigm: NA
|
Fear conditioning test |
20 weeks |
| |
|
Description: Mecp2 tg mice show increased freezing in response to context compared to wildtype controls, authors indicate enhanced hippocampal learning
Exp Paradigm: NA |
|
|
| Molecular profile |
Targeted expression1 |
Increased
Description: Mecp2 transgene shows increased expression equal to about 2 fold of the protein levels from wild type, this includes the human transgene and the endogenous protein
Exp Paradigm: NA
|
Western blot |
NA |
| |
|
Description: Mecp2 transgene shows increased expression equal to about 2 fold of the protein levels from wild type, this includes the human transgene and the endogenous protein
Exp Paradigm: NA |
|
|
| Emotion |
Anxiety1 |
No change |
Open field test |
Adult |
| Emotion |
Anxiety1 |
No change |
Light-dark exploration test |
Adult |
| Learning & memory |
Cued or contextual fear conditioning: memory of cue1 |
No change |
Fear conditioning test |
20 weeks |
| Motor phenotype |
General locomotor activity1 |
No change |
Open field test |
6-20 weeks |
| Motor phenotype |
Motor coordination and balance1 |
No change |
Accelerating rotarod test |
6 weeks |
| Neurophysiology |
Synaptic transmission1 |
No change |
Fepsp slope vs fiber volley amplitude |
NA |
|
| Not Reported: |
Circadian sleep/wake cycle, Communications, Immune response, Maternal behavior, Neuroanatomy / ultrastructure / cytoarchitecture, Physiological parameters, Repetitive behavior, Social behavior, |
