Circadian rhythm: protein expression periodicity

(AM100000)

Expression of proteins responsible for regulating the different phases of circadian cycle, e.g. Per1, CLOCK, cryptochrome-1

Liquid chromatography-mass spectrometry (LC-MS); Amylase release assay; Quantitative PCR (qRT-PCR); Immunohistochemistry; Immunoprecipitation; Western blot

Circadian rhythms: timing/phases of locomotor activity

(AM100001)

Timing of the different phases of circadian rhythm, including sleep and awakening, by monitoring initiation or cessation of locomotor activity

General observations; Beam crossing; Home cage behavior; Wheel running monitoring; Running wheel test; Electromyogram (EMG); Body temperature measurement

Locomotor activity in diurnal cycle

(AM100002)

Measurement of locomotor activity in home cage for a 12-hour light/12-hour dark cycle

General observations; Home cage behavior; Wheel running monitoring; Electromyogram (EMG); Open field test; Beam crossing

Non rapid eye movement sleep (NREMS)

(AM100004)

Frequency or duration of the sleep stages characterized by distinct EEG patterns, decreased metabolic activity, slowed breathing and heart rate, and the absence of rapid eye movement and dreaming. This is measured using NREMS bouts or bout lengths.

Electroencephalogram/electromyogram (EEG/EMG); Electroencephalogram (EEG); Polysomnography; Home cage behavior; Sleep analysis; Electromyogram (EMG)

Non rapid eye movement sleep (NREMS) pattern

(AM100005)

EEG patterns of different NREM sleep stages (i.e. 1, 2, 3 and 4)

Electroencephalogram (EEG); Electromyogram (EMG); Wheel running monitoring; Polysomnography; Home cage behavior; Sleep analysis

Rapid eye movement sleep (REMS)

(AM100006)

Frequency or duration of the sleep stage in which dreams occur and the body undergoes marked changes including rapid eye movement, loss of reflexes, and increased pulse rate and brain activity. This is measured using REMS bouts or bout lengths

Electroencephalogram/electromyogram (EEG/EMG); Electroencephalogram (EEG); Electromyogram (EMG); Polysomnography; Home cage behavior; Sleep analysis

Circadian rhythm

(AM100007)

Cycle from wakefulness through an orderly succession of sleep states and stages that occurs on an approximately 24-hour rhythm

Electroencephalogram/electromyogram (EEG/EMG); Wheel running monitoring; General observations; Electroencephalogram (EEG); Electromyogram (EMG); Immunostaining; Home cage behavior

Sleep pattern

(AM100008)

Duration or frequency of different sleep and wake stages

Electroencephalogram (EEG); Home cage behavior; Sleep analysis; Electromyogram (EMG); Body temperature measurement; Electroencephalogram/electromyogram (EEG/EMG); Running wheel test; Polysomnography

Sleep pattern: sleep latency

(AM100009)

Time between turning lights off and the onset of sleep

Beam crossing

Sleep pattern: total nighttime sleep

(AM100010)

Total time spent in nighttime sleep.

Beam crossing

Sleep pattern: sleep bout length

(AM100011)

Length of individual bouts of sleep.

Sleep analysis; Beam crossing

Sleep pattern: number of sleep bouts

(AM100012)

Number of sleep bouts.

Sleep analysis; Beam crossing

Sleep pattern: sleep recovery

(AM100013)

Measure of the efficiency of recovering lost sleep after a period of sleep deprivation.

Beam crossing

Sleep pattern: total daytime sleep

(AM100014)

Total time spent in daytime sleep

Beam crossing

Circadian rhythm: eclosion rhythm

(AM100015)

Eclosion profile analysis

Circadian rhythms: timing/phases of locomotor activity: morning activity

(AM100016)

Average or peak activity bouts, ususally measured by beam crossings, during the morning.

Beam crossing

Circadian rhythms: timing/phases of locomotor activity: evening activity

(AM100017)

Average or peak activity bouts, ususally measured by beam crossings, during the evening.

Beam crossing

Circadian rhythms: timing/phases of locomotor activity: night activity

(AM100018)

Average or peak activity bouts, ususally measured by beam crossings, during the night .

Beam crossing

Circadian rhythms: timing/phases of locomotor activity: evening activity onset

(AM100019)

Measure of early onset evening activity or delay in ensuing activity, ususally measured by beam crossings, during the early evening period (18:00-20:00) .

Beam crossing

Circadian rhythms: timing/phases of locomotor activity: midday activity

(AM100020)

Average or peak activity bouts, ususally measured by beam crossings, during the afternoon lull period/midday siesta (10:30-17:30).

Beam crossing

Locomotor activity in diurnal cycle: light phase

(AM100021)

Measurement of locomotor activity in home cage during the 12-hour light phase

Adaptation to dark phase

(AM100022)

adaptation of the circadian rhythm to disruptive stimuli such as restricted availability of food

Restricted feeding paradigm; Home cage behavior; Electromyogram (EMG); Wheel running monitoring; Open field test

Adaptation to circadian feeding behavior

(AM100023)

Adaptation of circadian rhythms to external disruptive stimuli such as restricted feeding times or lack of adaptation measured by increased mortality

Survival analysis

Rapid eye movement sleep: dark phase

(AM100025)

Frequency or duration of the sleep stage in which dreams occur and the body undergoes marked changes including rapid eye movement, loss of reflexes, and increased pulse rate and brain activity, during the 12 hour dark phase of the 24 hour diurnal cycle.

Electroencephalogram/electromyogram (EEG/EMG)

Rapid eye movement sleep: light phase

(AM100026)

Frequency or duration of the sleep stage in which dreams occur and the body undergoes marked changes including rapid eye movement, loss of reflexes, and increased pulse rate and brain activity, during the 12 hour light phase of the 24 hour diurnal cycle.

Electroencephalogram/electromyogram (EEG/EMG)